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109204 Giemsa's azur eosin methylene blue solution

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109204
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1092040100
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      Glass bottle 100 ml
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      1092040500
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          1092041000
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              Plastic bottle 1 l
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              1092041022
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                  Plastic bottle 20 PC
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                  1092042500
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                      Plastic bottle 2.5 l
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                      1092049025
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                          Barrel PE/met 25 l
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                          GT11092041002
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                              GT11092041022
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                                  1 PC
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                                  Description
                                  Catalogue Number109204
                                  DescriptionGiemsa's azur-eosin-methylene blue solution
                                  OverviewGiemsa’s Azure Eosin Methylene Blue solution - for microscopy, can be used for the staining of blood and bone marrow smears, paraffin sections and clinical-cytological specimens. Giemsa staining is one of the standard procedures in histology, demonstrating various cells with their morphological features better than with H&E stain. When used in hematological applications, Giemsa’s stain is frequently used in combination with other staining solutions, e. g. with May-Grünwald’s solution for Pappenheim (MGG) overview staining. This ready-to-use staining solution generally stains the nuclei red, based on the molecular interaction between the eosin Y dye and an Azure B-DNA complex. Both dyes assemble to an Eosin Y - Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. In histology and clinico-cytological applications, Giemsa’s staining without additional stainings is used as an extended overview staining method. In this method, the color of the various cell components is influenced by pretreatment of the specimen material. Here, chromatin-containing structures (e. g. cell nuclei) appear in various blue shades, while the acidophilic components show up in a variety of red shades. The 500 ml bottle is sufficient for 3500-5000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
                                  References
                                  Product Information
                                  HS Code3212 90 90
                                  Quality LevelMQ400
                                  Applications
                                  Applicationfor microscopy
                                  Biological Information
                                  Physicochemical Information
                                  Boiling point>65 °C (1013 hPa)
                                  Density0.99 g/cm3 (20 °C)
                                  Explosion limit5.5 - 44 %(V) Methanol
                                  Flash point18 °C
                                  Ignition temperature455 °C
                                  pH value6.0 - 8.2 (H₂O, 20 °C) (undiluted)
                                  Dimensions
                                  Materials Information
                                  Toxicological Information
                                  Safety Information according to GHS
                                  Hazard Pictogram(s) Hazard Pictogram(s)
                                  Hazard Pictogram(s)
                                  Hazard Pictogram(s)
                                  Hazard Statement(s)H225: Highly flammable liquid and vapour.
                                  H301 + H311 + H331: Toxic if swallowed, in contact with skin or if inhaled.
                                  H317: May cause an allergic skin reaction.
                                  H370: Causes damage to organs.
                                  Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
                                  P233: Keep container tightly closed.
                                  P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
                                  P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/doctor.
                                  P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
                                  P304 + P340 + P311: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor.
                                  P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
                                  Signal WordDanger
                                  Storage class3 Flammable liquids
                                  WGKWGK 2 obviously hazardous to water
                                  Disposal9
                                  Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
                                  Safety Information
                                  Categories of dangerhighly flammable, toxic
                                  Product Usage Statements
                                  Storage and Shipping Information
                                  StorageStore at +15°C to +25°C.
                                  Packaging Information
                                  Transport Information
                                  Declaration (railroad and road) ADR, RIDUN 1230 , 3 (6.1), II
                                  Declaration (transport by air) IATA-DGRUN 1230 , 3 (6.1), II
                                  Declaration (transport by sea) IMDG-CodeUN 1230 , 3 (6.1), II
                                  Supplemental Information
                                  Specifications
                                  Suitability for microscopypasses test
                                  Erythrocytesreddish-brownish
                                  Nuclei / Chromatinred to violet
                                  Eosinophilic granulesreddish to red-brown
                                  Neutrophilic granuleslight violet
                                  Lymphocyte cytoplasmnblue
                                  Monocyte cytoplasmgrey-blue
                                  Thrombocytesviolet
                                  Basophilic granulesdark violet
                                  Nuclei (Tissue section)blue to dark blue
                                  Cellsblue to dark blue
                                  Collagenpale blue
                                  Osteoidpale blue
                                  Eosinophilic granula (Tissue section)red
                                  Acidic mucopolysaccharidesreddish-violet
                                  Mast cell granulesreddish-violet
                                  Cartilage matrixreddish-violet
                                  Acidophilic materialsorange-red
                                  Helicobacter pylori
                                  blue to dark blue
                                  blue to dark blue
                                  Global Trade Item Number
                                  Catalogue Number GTIN
                                  1092040100 04022536111841
                                  1092040500 04022536111889
                                  1092041000 04022536720081
                                  1092041022 04022536707389
                                  1092042500 04022536111926
                                  1092049025 04022536111940
                                  GT11092041002 04061839134775
                                  GT11092041022 04061839134782

                                  Documentation

                                  Giemsa's azur eosin methylene blue solution SDS

                                  Title

                                  Safety Data Sheet (SDS) 

                                  Giemsa's azur eosin methylene blue solution Certificates of Analysis

                                  Product Number PackagingSpecificationLot Number
                                  1092040100 Glass bottlePDF Specification Document
                                  1092040500 Plastic bottlePDF Specification Document
                                  1092041000 Plastic bottlePDF Specification Document
                                  1092041022 Plastic bottlePDF Specification Document
                                  1092042500 Plastic bottlePDF Specification Document
                                  1092049025 Barrel PE/metPDF Specification Document
                                  GT11092041002 PDF Specification Document
                                  GT11092041022 PDF Specification Document

                                  User Guides

                                  Title
                                  User Guide (IFU) - 109204

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                                  Categories

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                                  IVD/OEM Materials & Reagents > Microscopy > Histology > Histological Staining Solutions
                                  IVD/OEM Materials & Reagents > Microscopy > Cytology > Cytological Staining Reagents
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