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MAB1217F Anti-FMC-7 B-Cell Lymphocyte Marker Antibody, clone FMC-7, FITC conjugated

This Anti-FMC-7 B-Cell Lymphocyte Marker Antibody, clone FMC-7, FITC conjugated is validated for use in Flow Cytometry (FC) for the detection of FMC-7 B-Cell Lymphocyte Marker.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: FMC-7, FMC7
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MAB1217F
100 assays  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMFITCMonoclonal Antibody
      Description
      Catalogue NumberMAB1217F
      ReplacesCBL1416F
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-FMC-7 B-Cell Lymphocyte Marker Antibody, clone FMC-7, FITC conjugated
      OverviewBIOCHEMISTRY: p I = 7.4
      Background InformationB cells are lymphocytes that play a large role in the humoral immune response as opposed to the cell-mediated immune response that is governed by T cells. The principal function of B cells is to make antibodies against soluble antigens. B cells are an essential component of the adaptive immune system. FMC-7 B-Cell Lymphocyte Marker is a 105 kDa B cell restricted antigen which is expressed on about 50% of adult human peripheral blood B cells. Upon in vivo B cell activation FMC7 expression initially increases. B cells involved in antibody secretion have lost the FMC7 determinant. The monoclonal antibody FMC7 delineates a subpopulation of B lymphocytes in normal blood. Expression of the antigen recognized by FMC7 appears to be maturation-linked, and it serves to distinguish different types of B cell leukemia.
      References
      Product Information
      FormatFITC
      Control
      • Human peripheral blood lymphocytes, HRIK and Raji cell lines
      PresentationThe antibody is supplied in 1.0 ml 0.1M Tris-HCl, 0.5M NaCl, 10mM glycine, pH 8.0, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-FMC-7 B-Cell Lymphocyte Marker Antibody, clone FMC-7, FITC conjugated is validated for use in Flow Cytometry (FC) for the detection of FMC-7 B-Cell Lymphocyte Marker.
      Key Applications
      • Flow Cytometry
      Application NotesThis antibody can be used for the differentiation of PLL from B-CLL. It is also useful in confirmation of the diagnosis of other disorders such as HCL, HCL-V and SLVL. Useful for the detection of residual disease in patients undergoing treatment for these disorders.


      SUGGESTED USAGE:

      Flow cytometry - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
      Biological Information
      ImmunogenThe human B-lymphoblastoid cell line, HRIK.
      CloneFMC-7
      HostMouse
      SpecificityThe FMC-7 antibody was not clustered at the 5th Leucocyte Typing Workshop held in Boston USA 1993. This antibody detects a glycoprotein of 105kD found on circulating B-lymphocytes (Brooks et al., 1981). Studies on normal lymphocytes, leukaemic cells and cell lines indicate that FMC-7 is a marker for a limited segment of the B cell maturation pathway (Catovsky et al., 1981; Zola et al., 1984). This glycoprotein is expressed in varying degrees on normal circulating B-cells, chronic B-cell leukaemia (B-CLL), prolymphocytic leukaemia (PLL) and other B cell neoplasias (Zola et al., 1984, 1987; Bloem et al., 1988; Melo et al., 1988; Ferro & Zola, 1990; Mulligan, 1990; Zola et al., 1991; Burthem & Cawley, 1994; Huh et al., 1994). It labels the same population as CD22 antibodies but reacts with a different antigen (Zola et al. 1987).

      Cell reactivity:1

      Stains peripheral blood B lymphocytes and tonsil B lymphocytes. No reaction with granulocytes, monocytes, platelets, erythrocytes, T lymphocytes or null cells. Reacts with HRIK and Raji cell lines.

      Clinical9,12,13 Expression

      B cell prolymphocytic leukemia (B-PLL) Strongly positive

      Hairy cell leukemia (HCL) Strongly positive

      Hairy cell leukaemia variant (HCL-V) Strongly positive

      Splenic lymphoma with villous lymphocytes (SLVL) Positive

      B cell chronic lymphocytic leukaemia (B-CLL) Negative to weakly positive
      IsotypeIgM
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • FMC-7
      • FMC7
      Molecular Weight105 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2 to 8°C, for up to 6 months. DO NOT FREEZE. Protect from light.

      WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB1217F 04053252666773

      Documentation

      Anti-FMC-7 B-Cell Lymphocyte Marker Antibody, clone FMC-7, FITC conjugated SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-FMC-7 B-Cell Lymphocyte Marker Antibody, clone FMC-7, FITC conjugated Certificates of Analysis

      TitleLot Number
      ANTI-FMC-7 B-CELL LYMPHOCYTE MARKER 2474924
      ANTI-FMC-7 B-CELL LYMPHOCYTE MARKER 3082469
      ANTI-FMC-7 B-CELL LYMPHOCYTE MARKER 2444103
      ANTI-FMC-7 B-CELL LYMPHOCYTE MARKER MONOCLONAL ANTIBODY, FITC CONJUGATED, READY TO USE - 2090120 2090120
      ANTI-FMC-7 B-CELL LYMPHOCYTE MARKER MONOCLONAL ANTIBODY, FITC CONJUGATED, READY TO USE - 2430436 2430436
      ANTI-FMC-7 B-CELL LYMPHOCYTE MARKER - 3156779 3156779
      ANTI-FMC-7 B-CELL LYMPHOCYTE MARKER - 3204245 3204245
      ANTI-FMC-7 B-CELL LYMPHOCYTE MARKER - 3238449 3238449
      ANTI-FMC-7 B-CELL LYMPHOCYTE MARKER - 3288667 3288667
      ANTI-FMC-7 B-CELL LYMPHOCYTE MARKER - 3327638 3327638
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