Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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BugBuster® Protein Extraction Reagent is formulated to gently disrupt the cell wall of E.coli and liberate soluble proteins. This flagship reagent from Merck utilizes a detergent mix that is capable of cell wall perforation without denaturing soluble protein. Simply add the BugBuster® Reagent to your cell pellet and incubate at room temperature for 10 minutes! No special preparation of your samples is required. No instruments are needed. Unlike other mechanical methods such as Sonication, protein denaturation is not a problem. BugBuster® reagent produces high yields that are reproducible, convenient and fast!
Product Advantages:
Standard reagent is supplied as a Tris-buffered "1X" ready-to-use liquid
BugBuster® reagent is superior to "Home Brew" lysis buffer and BugBuster® reagent with both Benzonase® nuclease and rLysozyme solution yielded lysates with the most 6XHis-CRP. (A) E.coli lysates (5μL of 1 mL total lysate) from various lysis protocols were fractionated and analyzed by SDS-PAGE. A band correspondinf to 6XHIS-CRP is prominently visualized in the BB +/+ lane.
BugBuster® Master Mixcombines BugBuster® Protein Extraction Reagent with Benzonase® Nuclease and rLysozyme Solution in one convenient reagent.
BugBuster® plus Benzonase® includes BugBuster® Protein Extraction Reagent and Benzonase® Nuclease as two separate components (500mL and 10 KU respectively) in a single kit.
BugBuster® Plus Lysonase includes BugBuster® Protein Extraction Reagent and Lysonase Bioprocessing Reagent, an optimized, ready-to-use blend of rLysozyme and Benzonase® Nuclease in a single kit.
BugBuster® 10Xis an unbuffered concentrated formulation of the BugBuster® Protein Extraction Reagent offering you the flexibility to customize your dilution and buffer composition.
