ZipTip® Pipette Tips
Concentrating and purifying samples for MALDI-ToF MS
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Concentrating and purifying samples for MALDI-ToF MS Less
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| Sample Preparation |
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| ZipTip® |
Categories
| Life Science Research > Protein Sample Preparation > Protein Concentration & Dialysis > Protein Concentrators |
ZipTip Advantages:
- Single-step desalting, concentration, and purification
- Fractionate complex samples for more meaningful data
- Ideal for peptides, proteins, nucleic acids, and more
- No dead volume for maximum recovery
- Eliminates time-consuming chromatography.
ZipTip is a 10 µL pipette tip with a 0.6 or 0.2 µL bed of chromatography media fixed at its end with no dead volume. It is ideal for concentrating and purifying samples for sensitive downstream analyses.
Examples of analyses enhanced by ZipTip sample preparation:
- Mass spectrometry
- HPLC (high performance liquid chromatography)
- Capillary electrophoresis
- Atomic absorption spectroscopy
The ZipTip pipette tip is simple and easy to use.
- Place the tip on a single- or multi-channel pipettor, standard 22-gauge blunt-end HPLC needle, or compatible automated liquid handling/sample preparation station.
- For sample binding, aspirate and dispense through the media several times.
- Similarly, aspirate and dispense appropriate wash solvent to remove contaminants.
- The concentrated, purified sample is eluted in 1–4 µL of compatible solvent with direct transfer to a mass spectrometer target, vial, or other analysis step.
For applications requiring smaller elution volumes (e.g., <1 µL), a micro-bed format containing 0.2 µL of media is available.
The ZipTip pipette tip provides a reproducible, high-recovery method for concentrating and purifying femtomoles to picomoles of peptides, proteins and oligonucleotides for improved analytical data. To simplify your analysis even further, you can, fractionate complex peptide mixtures by step elution.
Performance
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MALDI MS spectra of a tryptic peptide digest from an in-gel 2D digest. The top spectra represents a contaminated sample prior to sample clean-up. The lower spectra represents the sample after using a ZipTipC18 prior to MALDI TOF MS analysis.
Choose From 3 Different ZipTip Resins:
| Resin | Compatible Analytes |
| C18 Resin (0.6 µL or 0.2 µL bed volumes) | Oligonucleotides or small (<50 kDa) proteins/peptides in aqueous solution |
| C4 Resin | Proteins or peptides (3 kDa – 100 kDa) in aqueous solution |
| Strong cationic resin (SCX) | Peptides in solution with organic solvents or detergents |
The ZipTip® pipette tip is a 10 µL tip with a 0.6 or 0.2 µL bed of chromatography media fixed at its end. It is ideal for concentrating and purifying samples for sensitive analyses such as MALDI-ToF MS. The ZipTip® pipette tip provides a reproducible, high-recovery method for concentrating and purifying femtomoles to picomoles of peptides, proteins and oligonucleotides for improved analytical data. To simplify your analysis even further, you can fractionate complex peptide mixtures by step elution.
| Materials | ||
| Pipette Tip | Polypropylene | |
| C18 and µ-C18 Resin | Silica, 15 µm, 200 Å pore size | |
| C4 Resin | Silica, 15 µm, 300 Å pore size | |
| SCX Resin | Strong cation exchange, 12 µm, 300 Å pore size | |
| Volumes, µL | ||
| Pipette tip | 10 µL | |
| Resin Bed | 0.2 or 0.6 | |
| Capacity | ||
| ZipTipC18 | Typically 5 µg when used with saturating amounts of analyte | |
| ZipTipµ-C18 | Typically 2.0 µg when used with saturating amounts of analyte | |
| ZipTipC4 | Typically 3.3 µg when used with saturating amounts of analyte | |
| Dimensions | ||
| Tip Length | 31 mm | |
| Tip ID (top) | 3.375 mm | |
| Tip OD (top) | 5.8 mm | |
| Temperature, °C | 4至70 °C | |
| pH Range | pH1.5 – pH13.5; pH2 – pH12 for 24 hour exposure | |
Recommended Equipment
Manual Liquid Handling:
ZipTip pipette tips are compatible with most 10 µL single-channel pipettors. The Biohit Proline® electronic multi-channel pipettor is recommended for simultaneously processing eight samples.
Automated Liquid Handling:
ZipTip pipette tips are also proven for use on a variety of automated liquid handling systems including:
- ABI SymBiot® Sample Workstations for Biospectrometry™ with universal cone for ZipTip tips (ABI catalogue no. GEN602987) and rack adapter for ZipTip box
- Genomic Solutions ProMS™ Sample Preparation Station
- Bruker Daltonics MAP™ II and II/8 MALDI AutoPrep Systems with rack adapter for ZipTip box (Millipore catalogue no. ST0B DAP TR)
- Tecan GENESIS Workstation
- CyBio CyBi™-Well 96 Automated Pipettor