Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

111972 TSC (Tryptose Sulfite Cycloserine) Agar (base)

acc. ISO 15213, ISO 14189 and FDA-BAM GranuCult® prime

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: TSC-agar, Tryptose sulfite cycloserine agar

ph Value: 7.4 - 7.8 (H₂O, 25 °C) Solubility: 42 g/l
View Products on Sigmaaldrich.com
111972
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

ph ValueSolubility
7.4 - 7.8 (H₂O, 25 °C)42 g/l

Products

Catalogue NumberPackaging Qty/Pack
1119720500 Plastic bottle 500 g
Description
Catalogue Number111972
SynonymsTSC-agar, Tryptose sulfite cycloserine agar
OverviewGranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.
References
Product Information
HS Code3821 00 00
Quality LevelMQ200
Applications
Biological Information
Physicochemical Information
pH value7.4 - 7.8 (H₂O, 25 °C)
Bulk density660 kg/m3
Solubility42 g/l
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Statement(s)H319: Causes serious eye irritation.
Precautionary Statement(s)P264: Wash skin thoroughly after handling.
P280: Wear eye protection/ face protection.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P337 + P313: If eye irritation persists: Get medical advice/ attention.
Signal WordWarning
Storage class10 - 13 Other liquids and solids
WGKWGK 3 highly hazardous to water
Disposal3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Appearance (clearness)clear
Appearance (color)brown
Solidification behaviour (2 hrs., 45 °C)liquid
pH-value (25 °C)7.4 - 7.8
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Poured plate technique
Inoculum on reference medium (Clostridium perfringens ATCC 13124 (WDCM 00007))
Inoculum on reference medium (Clostridium perfringens ATCC 12916 (WDCM 00080))
Inoculum on reference medium (Clostridium perfringens ATCC 10543)
Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003))
≥ 10000
Colony count (Clostridium perfringens ATCC 13124 (WDCM 00007))
Colony count (Clostridium perfringens ATCC 12916 (WDCM 00080))
Colony count (Clostridium perfringens ATCC 10543)
Recovery on test medium (Clostridium perfringens ATCC 13124 (WDCM 00007))
≥ 70 %
Recovery on test medium (Clostridium perfringens ATCC 12916 (WDCM 00080))
≥ 70 %
Recovery on test medium (Clostridium perfringens ATCC 10543)
≥ 70 %
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003))
total inhibition
Blacking (Clostridium perfringens ATCC 13124 (WDCM 00007))
+
Blacking (Clostridium perfringens ATCC 12916 (WDCM 00080))
+
Blacking (Clostridium perfringens ATCC 10543)
+
Incubation: 20 ± 2 hours at (37 ± 1) °C anaerobic
Membrane filtration
Inoculum on reference medium (Clostridium perfringens ATCC 13124 (WDCM 00007))
Inoculum on reference medium (Clostridium perfringens ATCC 12916 (WDCM 00080))
Inoculum on reference medium (Clostridium perfringens ATCC 10543)
Inoculum on reference medium (Bacillus subtilis ATCC 6633 (WDCM 00003))
≥ 10000
Colony count (Clostridium perfringens ATCC 13124 (WDCM 00007))
Colony count (Clostridium perfringens ATCC 12916 (WDCM 00080))
Colony count (Clostridium perfringens ATCC 10543)
Recovery on test medium (Clostridium perfringens ATCC 13124 (WDCM 00007))
≥ 70 %
Recovery on test medium (Clostridium perfringens ATCC 12916 (WDCM 00080))
≥ 70 %
Recovery on test medium (Clostridium perfringens ATCC 10543)
≥ 70 %
Growth (Bacillus subtilis ATCC 6633 (WDCM 00003))
total inhibition
Blacking (Clostridium perfringens ATCC 13124 (WDCM 00007))
+
Blacking (Clostridium perfringens ATCC 12916 (WDCM 00080))
+
Blacking (Clostridium perfringens ATCC 10543)
+
Incubation: 21 ± 3 hours at (44 ± 1) °C anaerobic
A recovery rate of 70 % is equivalent to a productivity value of 0.7.
The indicated colony counts result from the sum of a triple determination.
Reference media: TSC-Agar
Fluorescence (Clostridium perfringens ATCC 13124 (WDCM 00007))+
Fluorescence (Clostridium perfringens ATCC 12916 (WDCM 00080))+
Incubation: 20 hrs. 37 °C.
Reference: TSC-agar for Cl.perfingens; Blood-agar for all other strains.
Global Trade Item Number
Catalogue Number GTIN
1119720500 04022536137773

Related Products & Applications

Accessories (Primary)

Catalogue Number Description
100888 Clostridium perfringens selective supplement
107040 Petri-dish rack
113203 UV lamp 366 nm
114226 Anaeroclip®

Accessories (Secondary)

Catalogue Number Description
100888 Clostridium perfringens selective supplement

Related Products By: Brand Facete

GranuCult™

Categories

Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media > Enrichment, Isolation & Differentiation by Organism > Bacillus
Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media > Enrichment, Isolation & Differentiation by Organism > Yeast & Molds
Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media
Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media > Enrichment & Microbial Enumeration > Plate Count
Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Organism > Bacillus Cereus
Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Product > Dehydrated and Ready-to-use Culture Media
Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Organism > Clostridium Perfringens
Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media > Enrichment, Isolation & Differentiation by Organism > Clostridia & Sulfite reducing anaerobic Bacteria