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616407 TACE Substrate IV, Fluorogenic - Calbiochem

TACE Substrate IV, Fluorogenic - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: TNF-α Convertase Enzyme Substrate IV, Fluorogenic, Abz-LAQAVRSSSR-Dpa, ADAM-17 Substrate IV, Fluorogenic

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616407
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
616407-1MG Glass bottle 1 mg
Description
OverviewAn internally-quenched fluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity and high-throughput screening of TACE inhibitors (kcat = 21.6 s-1 and substrate concentration at half the Vmax, S0.5 = 342 µM). The substrate is cleaved at Ala-Val amide bond. Acts as a poor substrate for MMP-1, MMP-9, and MMP-13. Displays better solubility in aqueous buffers.
Catalogue Number616407
Brand Family Calbiochem®
SynonymsTNF-α Convertase Enzyme Substrate IV, Fluorogenic, Abz-LAQAVRSSSR-Dpa, ADAM-17 Substrate IV, Fluorogenic
References
ReferencesJin, G., et al. 2002. Anal. Biochem. 302, 269.
Product Information
ATP CompetitiveN
FormYellow lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₅₉H₉₃N₂₃O₂₀
Chemical formulaC₅₉H₉₃N₂₃O₂₀
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetFluorogenic decapeptide substrate that is useful for continuous monitoring of TACE activity
Primary Target IC<sub>50</sub>kcat = 21.6 s-1 and substrate concentration at half the Vmax, S0.5 = 342 µM
Purity≥95% by HPLC
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Peptide SequenceAbz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
616407-1MG 04055977185713

Documentation

TACE Substrate IV, Fluorogenic - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

TACE Substrate IV, Fluorogenic - Calbiochem Certificates of Analysis

TitleLot Number
616407

References

Reference overview
Jin, G., et al. 2002. Anal. Biochem. 302, 269.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-December-2018 JSW
SynonymsTNF-α Convertase Enzyme Substrate IV, Fluorogenic, Abz-LAQAVRSSSR-Dpa, ADAM-17 Substrate IV, Fluorogenic
DescriptionAn internally quenched, fluorogenic, 10-amino acid peptide substrate that is useful for monitoring TNF-α Convertase Enzyme (TACE) activity by a continuous fluorometric assay. Also useful for screening TACE inhibitors (kcat = 21.6 s-1 when the substrate concentration is half the Vmax, S0.5 = 342 µM). The substrate is cleaved at the Ala-Val amide bond. The substrate is not substantially cleaved by MMP-1, MMP-9, or MMP-13. Displays improved solubility in aqueous buffers. Excitation max: ~320 nm; Emission max: ~420 nm.
FormYellow lyophilized solid
FormulationSupplied as a trifluoroacetate salt.
Recommended reaction conditions50 mM Tris-HCl, pH 7.4, 25 mM NaCl, 4% glycerol.
Chemical formulaC₅₉H₉₃N₂₃O₂₀
Peptide SequenceAbz-Leu-Ala-Gln-Ala-Val-Arg-Ser-Ser-Ser-Arg-Dpa
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml)
Storage Protect from moisture
Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesJin, G., et al. 2002. Anal. Biochem. 302, 269.