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Steriflip® Filter Units

No sample transfer, just connect your tube and filter

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Overview

Specifications

Ordering Information

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Units with fast flow and low protein binding Millipore Express (PES) membrane · Catalogue No.Clear Sorting & Filtering Show Filter
Catalogue NumberDescriptionPore SizePack Size
SCGP00525Steriflip-GP Sterile Centrifuge Tube Top Filter Unit 0.22 µm 25

Each box of 25 filter units includes one centrifuge tube stand

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Units with very low protein binding Durapore (PVDF) membraneClear Sorting & Filtering Show Filter
Catalogue NumberDescriptionPore SizePack Size
SE1M179M6Steriflip-GV Sterile Centrifuge Tube Top Filter Unit 0.22 µm 25
SE1M003M00Steriflip-HV Sterile Centrifuge Tube Top Filter Unit 0.45 µm 25

Each box of 25 filter units includes one centrifuge tube stand

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Unit with nylon net filterClear Sorting & Filtering Show Filter
Catalogue NumberDescriptionPore SizePack Size
SCNY00020Steriflip Sterile Centrifuge Tube Top Filter Unit 20.0 µm 25
SCNY00040Steriflip Sterile Centrifuge Tube Top Filter Unit 40.0 µm 25
SCNY00060Steriflip Sterile Centrifuge Tube Top Filter Unit 60.0 µm 25
SCNY00100Steriflip Sterile Centrifuge Tube Top Filter Unit 100.0 µm 25

Each box of 25 filter units includes one centrifuge tube stand

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AccessoriesClear Sorting & Filtering Show Filter
Catalogue NumberDescriptionPack Size
SC50FL025Steriflip Funnel Attachment, non-sterile 25

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Documentation

References

Reference overview
Novel Single-Cell-Level Phenotypic Assay for Residual Drug Susceptibility and Reduced Replication Capacity of Drug Resistant Human Immunodeficiency Virus Type 1
Haili Zhang, Yan Zhou, Cecily Alcock, Tara Kiefer, Daphne Monie, Janet Siliciano, Quan Li, Paul Pham, Joseph Cofrancesco, Deborah Persaud, and Robert F. Siliciano; Journal of Virology, February 2004, p. 1718-1729, Vol. 78, No. 4.
Journal of Virology, February 2004, p. 1718-1729, Vol. 78, No. 4.  2004

FAQ

QuestionAnswer
Should I filter my acrylamide solution prior to gel casting?Yes, filtering your acrylamide solution before adding the polymerization activator ammonium persulfate and TEMED will accomplish two things.
1. It will eliminate particulate matter that may interfere with base calling when sequencing or that may create "hot" spots in the gel, and
2. It will aid in degassing, which will promote efficient polymerization of the gel.

Products appropriate for this application would be Stericup, Steritop or Steriflip with express membrane.
Is there a stand for the Steriflip?Yes. If you would prefer to put the Steriflip unit into a stand when vacuum filtering your sample rather then holding it , you can order catalog number SC50BS001.
While filtering serum through my Steriflip the solution appears to be bubbling what can I do?Because Steriflip is a closed system, there is a small upstream vent that will bubble air slowly through the solution before it is filtered. If the foaming is an objection, you may purchase SC50FL025 that is a package of 25 non-sterile funnels. The funnel attaches instead of the tube, then you can pour the solution through into the funnel and turn on the vacuum. The solution will be filtered into the receiving tube.
What is Steriflip?Steriflip is a sterile vacuum-driven device for use with 50 ml centrifuge tubes. The filter unit incorporates Millipore's 0.22um Express (PES) membrane.
What tubes will work with my Steriflip?The Steriflip comes with one sterile polypropylene 50 ml tube that you filter your sample into, but you must supply your own starting tubes. The unit will fit onto most standard double start (double lead ) 50 ml tubes. Millipore sells these tubes in 100 and 500 packs.
The following chart lists some manufacturers and numbers of Steriflip compatible tubes:
MilliporeSC50TB100, SC50TB500
Corning25325-50, 25335-50
Falcon2070, 2098
Iwaki2330-050, 2331-050, 2341-050
Nunc334959, 334940
Perfector Scientific 2650
What is the shelf life on the Steriflip?The shelf life on the Steriflip is two years from the date of manufacture. The expiration date will be on the package when you receive it.
What is the typical hold-up volume of the of the Steriflip?The typical hold-up volume of the Steriflip is 600ul.
Do you have a filter for 100% serum?Yes, you can filter serum with Millipore Express membrane. The Express membrane was designed for this application and offers fast filtration time. Millipore Express is available in a large number of filter devices including Millex syringe filters, Stericup and Steritop vacuum devices, Sterivac for large volumes of media and Steriflip for 50 mls.
Is the Express membrane surfactant free?Yes, the Express membrane is surfactant free. It also has a very low extractables specification - less then 1.5% by weight.
Is the Steriflip autoclavable?No. Steriflips are provided to you pre-sterilized and individually wrapped. The sterilization method is gamma irradiation.

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Categories

Life Science Research > Cell Culture and Systems > Sterile Filtration Devices