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531546 STAT3 Inhibitor XX, inS3-54 - CAS 328998-92-1 - Calbiochem

A cell permeable compound that selectively binds to STAT3 and inhibits its DNA binding activity (IC₅₀ = 13.8 µM) and reduces the expression of STAT3 dependent genes.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: (E)-4-(3-(4-nitrobenzylidene)-2-oxo-5-phenyl-2,3-dihydro-1H-pyrrol-1-yl)benzoic acid

Primary Target: STAT3
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
328998-92-1C₂₄H₁₆N₂O₅

Products

Catalogue NumberPackaging Qty/Pack
5315460001 Glass bottle 10 mg
Description
OverviewA cell permeable pyrrolonyl benzoic acid derivative that selectively and non-covalently binds to STAT3 and inhibits its DNA binding activity in a dose and time dependent manner (IC50 = 13.8 µM after 29 hours incubation) and reduces the expression of STAT3 dependent genes (Cyclin D1, survivin, VEGF, MMP-2, MMP-9, and Twist) in A549 and MDA-MB-231 cells. However, it does not affect STAT3 dimerization or binding to the SH2 domain and has no effect on total STAT3 or basal level of Tyr705 phosphorylated STAT3. Preferentially induces apoptosis in cancer cells (A549 and MDA-MB-468) and inhibits their survival, but has much reduced effect on non-cancer IMR90 lung fibroblasts or MCF10A1 mammary epithelial cells. Shown to block cancer cell migration and invasion in a dose- and time-dependent manner.

Please note that the molecular weight for this compound is batch-specific due to variable water content.
Catalogue Number531546
Brand Family Calbiochem®
Synonyms(E)-4-(3-(4-nitrobenzylidene)-2-oxo-5-phenyl-2,3-dihydro-1H-pyrrol-1-yl)benzoic acid
References
ReferencesHuang, W., et al. 2014. ACS Chem. Bio. 9, 1188.
Product Information
CAS number328998-92-1
FormOrange powder
Hill FormulaC₂₄H₁₆N₂O₅
Chemical formulaC₂₄H₁₆N₂O₅
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary TargetSTAT3
Primary Target IC<sub>50</sub>13.8 µ
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
5315460001 04055977287264

Documentation

STAT3 Inhibitor XX, inS3-54 - CAS 328998-92-1 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Huang, W., et al. 2014. ACS Chem. Bio. 9, 1188.

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-March-2015 JSW
Synonyms(E)-4-(3-(4-nitrobenzylidene)-2-oxo-5-phenyl-2,3-dihydro-1H-pyrrol-1-yl)benzoic acid
DescriptionA cell permeable pyrrolonyl benzoic acid derivative that selectively and non-covalently binds to STAT3 and inhibits its DNA binding activity in a dose and time dependent manner (IC50 = 13.8 µM after 29 hours incubation) and reduces the expression of STAT3 dependent genes (Cyclin D1, survivin, VEGF, MMP-2, MMP-9, and Twist) in A549 and MDA-MB-231 cells. However, it does not affect STAT3 dimerization or binding to the SH2 domain and has no effect on total STAT3 or basal level of Tyr705 phosphorylated STAT3. Preferentially induces apoptosis in cancer cells (A549 and MDA-MB-468) and inhibits their survival, but has much reduced effect on non-cancer IMR90 lung fibroblasts or MCF10A1 mammary epithelial cells. Shown to block cancer cell migration and invasion in a dose- and time-dependent manner.
FormOrange powder
Intert gas (Yes/No) Packaged under inert gas
CAS number328998-92-1
Chemical formulaC₂₄H₁₆N₂O₅
Purity≥98% by HPLC
SolubilityDMSO (50 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesHuang, W., et al. 2014. ACS Chem. Bio. 9, 1188.