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2102 Protein-Concentrate Kit (Med)

2102
1 kit  1 kit
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
Protein Concentration
Description
Catalogue Number2102
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionProtein-Concentrate Kit (Med)
OverviewThe Chemicon Protein-Concentrate kit uses a proprietary reagent Universal Protein Precipitation Agent (UPPA). Protein solutions as dilute as 1 ng/mL can be quantitatively concentrated into a small volume. Protein precipitation is not effected by the presence of detergents, chaotropic, or other common laboratory agents. After precipitation, the precipitate is suspended in a small volume of Precipitate Suspension Buffer supplied with each kit. If the protocol is followed correctly, the recovery is generally 100%. There are enough reagents for concentrating up to 30 mL of dilute protein solution.
Materials Required but Not DeliveredCentrifuge

Centrifuge tubes

Microfuge

Spin columns
References
Product Information
Components
  • UPPA-I 100 mL
  • UPPA-II 100 mL
  • OrgoSol 2 x 50 mL
  • UPC-SEED 2 x 0.30 mL
  • Precipitate Buffer I 2 x 2.0 mL
  • Precipitate Buffer II 2 x 0.5 mL
  • UCP-Wash 2 x 2.0 mL
Applications
Key Applications
  • Protein Concentration
Application NotesSuitable for concentrating proteins for running gels, raising antibodies, protein purification, protein assays and other applications.

This kit is not suitable for those proteins which may lose some of their biological activities when precipitated.

PROTOCOL: ImportantPerform the entire procedure in the cold (ice bucket) unless specified otherwise. Concentration should be performed in a centrifuge tube. For small volumes, use microfuge tubes. Always position microfuge tubes in the centrifuge at the same orientation, i.e. cap-hinge facing out-ward, this will allow the pellet to remain glued to the same side of the tube during repeated centrifugations and minimize the loss of protein pellets. See product insert for protocol.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at room temperature for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically.
Packaging Information
Material Size1 kit
Material Package1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
2102 04053252268694

Documentation

Protein-Concentrate Kit (Med) SDS

Title

Safety Data Sheet (SDS) 

Protein-Concentrate Kit (Med) Certificates of Analysis

TitleLot Number
PROTEIN-CONCENTRATE KIT (MED) - 2055952 2055952
PROTEIN-CONCENTRATE KIT (MED) - 2187332 2187332
PROTEIN-CONCENTRATE KIT (MED) - 2290361 2290361
PROTEIN-CONCENTRATE KIT (MED) - 3194820 3194820
PROTEIN-CONCENTRATE KIT (MED) - 3702002 3702002
PROTEIN-CONCENTRATE KIT (MED) - 3802827 3802827
PROTEIN-CONCENTRATE KIT (MED) - 3862859 3862859
PROTEIN-CONCENTRATE KIT (MED) - 3995674 3995674
PROTEIN-CONCENTRATE KIT (MED) -2500387 2500387
PROTEIN-CONCENTRATE KIT (MED) -2689043 2689043

Data Sheet

Title
PROTEIN-CONCENTRATE KIT (MED)