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537059 Propidium Iodide - CAS 25535-16-4 - Calbiochem

Membrane impermeable DNA intercalator.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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537059
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
25535-16-4C₂₇H₃₄N₄I₂

Products

Catalogue NumberPackaging Qty/Pack
537059-100MG Glass bottle 100 mg
537059-1GM Plastic ampoule 1 gm
537059-250MG Plastic ampoule 250 mg
537059-50MG Glass bottle 50 mg
Description
OverviewMembrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for flow cytometry for staining apoptotic cells and nuclei. Can be used to differentiate between apoptotic and necrotic cell death while stains only necrotic cells. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate (FITC).
Extinction coefficient(493 nm): 5900 M -1cm-1;(287 nm): 55,700 M -1cm-1;
Catalogue Number537059
Brand Family Calbiochem®
References
ReferencesBelloc, F., et al. 1994. Cytometry 17, 59.
Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods 154, 11.
Pollice, A.A., et al. 1992. Cytometry 13, 432.
Product Information
CAS number25535-16-4
ATP CompetitiveN
FormDark red solid
Hill FormulaC₂₇H₃₄N₄I₂
Chemical formulaC₂₇H₃₄N₄I₂
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Primary TargetMembrane impermeable DNA intercalator
Sample TypeIntact Cells
Physicochemical Information
Cell permeableN
Emission max.
Excitation max.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSSF7949600
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
537059-100MG 04055977195897
537059-1GM 04055977195880
537059-250MG 04055977195903
537059-50MG 04055977195910

Documentation

Propidium Iodide - CAS 25535-16-4 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Propidium Iodide - CAS 25535-16-4 - Calbiochem Certificates of Analysis

TitleLot Number
537059

References

Reference overview
Belloc, F., et al. 1994. Cytometry 17, 59.
Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods 154, 11.
Pollice, A.A., et al. 1992. Cytometry 13, 432.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Stem Cell Resource Brochure GBP

Citations

Title
  • Oliveira, J.B., et al. 2007. Procedings of the National Academy of Science 104, 8953.
  • Tirado, O.M., et al. 2005. Cancer Research 65, 9320.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision29-August-2008 RFH
    DescriptionMembrane impermeable DNA intercalator. Has red fluorescence at 488 nm. Useful for staining apoptotic cells and nuclei by flow cytometry. Can be used to differentiate between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes labeled with fluorescein isothiocyanate. Has the following spectral properties: extinction coefficient: 5,900 M-1cm-1 (493 nm); 55,700 M-1cm-1 (287 nm); 13,800 M-1cm-1 (252.5 nm); excitation maximum: 536 nm; emission maximum: 617 nm.
    FormDark red solid
    CAS number25535-16-4
    RTECSSF7949600
    Chemical formulaC₂₇H₃₄N₄I₂
    Structure formulaStructure formula
    SolubilityH₂O. Solubility may be improved by first dissolving in a very small amount of DMSO, then adding material to an aqueous solution.
    Storage Protect from light
    +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
    Toxicity Irritant
    ReferencesBelloc, F., et al. 1994. Cytometry 17, 59.
    Crompton, T., et al. 1992. Biochem. Biophys. Res. Commun. 183, 532.
    Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
    de Caestecker, M.P., et al. 1992. J. Immunol. Methods 154, 11.
    Pollice, A.A., et al. 1992. Cytometry 13, 432.
    Citation
  • Oliveira, J.B., et al. 2007. Procedings of the National Academy of Science 104, 8953.
  • Tirado, O.M., et al. 2005. Cancer Research 65, 9320.
    		•