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528283 Polo-like Kinase Inhibitor II, BTO-1 - CAS 40647-02-7 - Calbiochem

The Polo-like Kinase Inhibitor II, BTO-1, also referenced under CAS 40647-02-7, controls the biological activity of Polo-like Kinase. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 5-Cyano-7-nitro-2-(benzothiazolo-N-oxide)-carboxamide

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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
40647-02-7C₉H₄N₄O₄S

Products

Catalogue NumberPackaging Qty/Pack
528283-2MG Plastic ampoule 2 mg
Description
OverviewA cell-permeable benzothiazolo-N-oxide compound that targets the ATP-binding pocket of polo-like kinase and is shown to inhibit Plk1 kinase activity in cell-free kinase assays (IC50 = 8.0 µM) and suppress the phosphorylation of cellular Plk1 substrate Cdc25C in rat kangaroo kidney-derived PTK cells (75% inhibition at 6.3 µM). BTO-1 treatment of cultured cells results in mitosis defects consistent with loss-of-Plk1 phenotypes seen in Plk1 RNAi-treated U20S cells, including the appearance of monopolar spindles and the reduction of γ-tubulin at the centrosomes. Plk1 inhibition by BTO-1 in HeLa cells results in a blockage of Rho and Rho-GEF recruitment, which is essential for the assembly of a functional contractile ring.
Catalogue Number528283
Brand Family Calbiochem®
Synonyms5-Cyano-7-nitro-2-(benzothiazolo-N-oxide)-carboxamide
References
ReferencesBrennan, I.M., et al. 2007. PloS ONE 2, e409.
Peters, U., et al. 2006. Nat. Chem. Biol. 2, 618.
McInnes, C., et al. 2006. Nat. Chem. Biol. 2, 608.
Product Information
CAS number40647-02-7
FormBeige to brown solid
Hill FormulaC₉H₄N₄O₄S
Chemical formulaC₉H₄N₄O₄S
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity≥97% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 20/21/22-36/37/38

Harmful by inhalation, in contact with skin and if swallowed.
Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36/37

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing and gloves.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
528283-2MG 04055977196580

Documentation

Polo-like Kinase Inhibitor II, BTO-1 - CAS 40647-02-7 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Polo-like Kinase Inhibitor II, BTO-1 - CAS 40647-02-7 - Calbiochem Certificates of Analysis

TitleLot Number
528283

References

Reference overview
Brennan, I.M., et al. 2007. PloS ONE 2, e409.
Peters, U., et al. 2006. Nat. Chem. Biol. 2, 618.
McInnes, C., et al. 2006. Nat. Chem. Biol. 2, 608.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision30-August-2016 JSW
Synonyms5-Cyano-7-nitro-2-(benzothiazolo-N-oxide)-carboxamide
DescriptionA cell-permeable benzothiazolo-N-oxide compound that targets the ATP-binding pocket of polo-like kinase and is shown to inhibit Plk1 kinase activity in cell-free kinase assays (IC50 = 8.0 µM) and suppress the phosphorylation of cellular Plk1 substrate Cdc25C in rat kangaroo kidney-derived PTK cells (75% inhibition at 6.3 µM). BTO-1 treatment of cultured cells results in mitosis defects consistent with loss-of-Plk1 phenotypes seen in Plk1 RNAi-treated U20S cells, including the appearance of monopolar spindles and the reduction of γ-tubulin at the centrosomes. Plk1 inhibition by BTO-1 in HeLa cells results in a blockage of Rho and Rho-GEF recruitment, which is essential for the assembly of a functional contractile ring.
FormBeige to brown solid
Intert gas (Yes/No) Packaged under inert gas
CAS number40647-02-7
Chemical formulaC₉H₄N₄O₄S
Structure formulaStructure formula
Purity≥97% by HPLC
SolubilityDMSO (1 mg/ml)
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Harmful
ReferencesBrennan, I.M., et al. 2007. PloS ONE 2, e409.
Peters, U., et al. 2006. Nat. Chem. Biol. 2, 618.
McInnes, C., et al. 2006. Nat. Chem. Biol. 2, 608.