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GF142 Platelet Derived Growth Factor-AA Protein, Recombinant human

The Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture UniProt Number: P04085 Entrez Gene Number: NM_002607.4, NM_033023.3
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GF142
10 µg  
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Overview

Key Spec Table

Key ApplicationsSpeciesSourcePurity
CULTHuman E.coliGreater than 98% by SDS-PAGE and HPLC analysis.
Description
Catalogue NumberGF142
ReplacesGF017
DescriptionPlatelet Derived Growth Factor-AA Protein, Recombinant human
OverviewPlatelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AA is a 28.5 kDa A chain homodimer protein containing 250 amino acid residues.
Alternate Names
  • PDGF-AA
Product Information
PresentationLyophilized from ddH2O.
Quality LevelMQ100
Applications
ApplicationThe Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.
Key Applications
  • Cell Culture
Application NotesFor most in vitro applications, PDGF-AA exerts its biological activity in the concentration range of 0.2 to 10.0 ng/mL. Responding cells are (partial list): Fibroblasts, connective tissue.
Biological Information
PurityGreater than 98% by SDS-PAGE and HPLC analysis.
SourceE.coli
Specific ActivityHuman PDGF-AA is fully biologically active when compared to standards, as determined by colorimetric assay using BALB / 3T3 cells.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; knockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene.
Gene Symbol
  • PDGFA
  • PDGF-1
  • PDGF-A
  • PDGF1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P04085 # Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.
SIZE: 211 amino acids; 24043 Da
SUBUNIT: Antiparallel disulfide-linked dimer of non-identical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. Interacts with CSPG4.
SUBCELLULAR LOCATION: Secreted.
DOMAIN: SwissProt: P04085 The long form contains a basic insert which acts as a cell retention signal.SIMILARITY: Belongs to the PDGF/VEGF growth factor family.
MISCELLANEOUS: A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor.
Stem Cell Type
  • Neural Stem Cells
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe lyophilized PDGF-AA is stable for a few weeks at room temperature, but best stored at -80°C. After a quick spin, reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into aqueous buffered solutions and stored at 2-8°C for one week or -20°C for future use. Reconstituted PDGF-AA should be stored in undiluted aliquots at -20°C for up to six months.
Packaging Information
Material Size10 µg
Global Trade Item Number
Catalogue Number GTIN
GF142 04053252271618