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33-126 PIP5-Kinase HTRF Assay

The PIP5 Kinase HTRF Assay kit provides a method for assaying PI(4)P-5 kinases in a homogenous 1920 well format. Suitable for screening of potential inhibitors of these enzymes.

PIP5-Kinase HTRF Assay MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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33-126
1920 wells  Kit capacity: 1920 wells
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Overview

Description
Catalogue Number33-126
Brand Family Upstate
Trade Name
  • HTRF
  • Upstate
DescriptionPIP5-Kinase HTRF Assay
Product Information
Components
  • Reaction Buffer (4X)
  • DM A Detection Reagent
  • DM C Detection Reagent
  • DM E Detection Reagent
  • Phosphatidylinositol 4-phosphate (1mM)
  • Phosphatidylserine (1mM)
  • Stop Reagent D
Detection methodHTRF
Quality LevelMQ100
Applications
ApplicationThe PIP5 Kinase HTRF Assay kit provides a method for assaying PI(4)P-5 kinases in a homogenous 1920 well format. Suitable for screening of potential inhibitors of these enzymes.
Key Applications
  • Kinase Assay
Biological Information
Species Reactivity
  • Human
  • Mouse
Analytes Available
  • PIP5 Kinase
Entrez Gene Number
Entrez Gene SummaryPhosphatidylinositol 4-phosphate 5-kinase type-1 alpha
catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs.
Gene Symbol
  • PIP5K1α
  • PIP5K1γ
Target Sub-FamilyPI3K/PI4K
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions12 months at -70°C
Packaging Information
Material Size1920 wells
Material PackageKit capacity: 1920 wells
Global Trade Item Number
Catalogue Number GTIN
33-126 04053252374739