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FCMAB100P Sigma-AldrichMilli-Mark Anti-Phospho-Erk1/2 Antibody (Thr202/Tyr204, Thr185/Tyr187)-PE

This Milli-Mark Phospho-Erk1/2 antibody is validated for use in Flow & WB for the detection of the Milli-Mark Phospho-Erk1/2 protein.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

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Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: Erk1, Erk2, p44-MAPK, p42-MAPK, MAPK2, PRKM2, p41mapk, PRKM1, p44, ERK2, P42MAPK, ERK, p42, MAPK1, ERT1, ERT2, Mitogen-activated protein kinase, MAP Kinase 2, MAP Kinase 1, MAPK UniProt Number: P28482

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Overview

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	FC, WB	Rb	PE	Monoclonal Antibody

Description
Catalogue Number	FCMAB100P
Trade Name	Milli-Mark
Description	Milli-Mark Anti-Phospho-Erk1/2 Antibody (Thr202/Tyr204, Thr185/Tyr187)-PE
Alternate Names	Erk1 Erk2 p44-MAPK p42-MAPK MAPK2 PRKM2 p41mapk PRKM1 p44 ERK2 P42MAPK ERK p42 MAPK1 ERT1 ERT2 Mitogen-activated protein kinase MAP Kinase 2 MAP Kianse 1 MAPK
Background Information	Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as Erk1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator of the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs), GPCR, and/or integrin stimulation. This activates the Ras-Raf-MEK-Erk pathway that results in the phosphorylation/activation of Erk1/2 (p44/p42) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 & Erk2, respectively).

Product Information
Format	PE
Control	Jurkat Cells
Presentation	Purified Rabbit monoclonal supernatant conjugated to phycoerythrin in buffer containing PBS with 0.05% sodium azide
Quality Level	MQ100

Applications
Application	This Milli-Mark Phospho-Erk1/2 antibody is validated for use in Flow & WB for the detection of the Milli-Mark Phospho-Erk1/2 protein.
Key Applications	Flow Cytometry Western Blotting

Biological Information
Immunogen	Phosphorylated recombinant peptide corresponding to amino acids surrounding the pTEpY motif in the activation loop of human phospho-Erk1/2, in which the Thr and Tyr residues are phosphorylated
Epitope	Phosphorylated Thr185/Phosphorylated Tyr187
Clone	AW39R
Host	Rabbit
Specificity	Antibody recognizes Recognizes Erk 1 & 2 only when dually phosphorylated on its TxY activation motif.
Isotype	IgG
Species Reactivity	Human Mouse Rat
Species Reactivity Note	Tested and passed on Human. based on 100% sequence homology.
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_002736.3
Entrez Gene Summary	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Gene Symbol	Erk1 Erk2 p44-MAPK p42-MAPK MAPK2 PRKM2 p41mapk PRKM1 p44 ERK2 P42MAPK ERK p42 MAPK1 ERT1 ERT2 Mitogen-activated protein kinase MAP Kinase 2 MAP Kinase 1 MAPK
Modifications	Phosphorylation
Purification Method	Protein A Purfied
UniProt Number	P28482
UniProt Summary	FUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). COFACTOR: Magnesium (By similarity). SIZE: 360 amino acids; 41390 Da SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4. DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight	42kDa and 44kDa Observed

Product Usage Statements
Quality Assurance	Evaluated by Flow Cytometry with Jurkat cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 6 months at 2-8°C from date of receipt. Protect from light.

Packaging Information
Material Size	100 tests

Global Trade Item Number
Catalogue Number	GTIN
FCMAB100P	04053252734014

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