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FCMAB103AP Milli-Mark Anti-Ki67 Antibody, clone Ki-S5 APC conjugate

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FCMAB103AP
100 tests  
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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, ICC, IHC, FC, IH(P)MAPCMonoclonal Antibody
Description
Catalogue NumberFCMAB103AP
Trade Name
  • Milli-Mark
DescriptionMilli-Mark Anti-Ki67 Antibody, clone Ki-S5 APC conjugate
Alternate Names
  • MKI67
    KIA
    Ki-67
Background InformationKi67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a marker of cell cycling and proliferation. This specificity of Ki-S5 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in tumors such as Non-Hodgkin's lymphoma (Kreipe et al., 1993), mammary carcinomas (Mauri et al., 1984), ovarian tumors (Ballin et al., 1994) and prostate cancer.
Product Information
FormatAPC
Control
  • Stimulated Jurkat Cells
PresentationPurified mouse monoclonal IgG1 conjugated to Allophycocyanin (APC) in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationDetect Ki67 using this Milli-Mark Anti-Ki67 Antibody, clone Ki-S5 APC conjugate validated for use in FC, IC, IH, FC, IH(P).
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
  • Flow Cytometry
  • Immunohistochemistry (Paraffin)
Biological Information
ImmunogenNuclear protein preparation from the human cell line U937.50
CloneKi-S5
HostMouse
SpecificityAntibody recognizes Ki67.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • MKI67
  • KIA
  • Ki-67
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P46013 # Thought to be required for maintaining cell proliferation.
SIZE: 3256 amino acids; 358694 Da
SUBUNIT: Binds through the FHA domain to MKI67IP.
SUBCELLULAR LOCATION: Nucleus. Note=Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
DEVELOPMENTAL STAGE: Expression of this antigen occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected.
SIMILARITY: SwissProt: P46013 ## Contains 1 FHA domain. ... hide » see more »
Molecular Weight345 & 395 kDa observed
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry with Jurkat cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8oC in undiluted aliquots for up to 6 months from date of receipt. Protect from light.
Packaging Information
Material Size100 tests
Global Trade Item Number
Catalogue Number GTIN
FCMAB103AP 04053252471735