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FCMAB314F Milli-Mark Anti-CNPase-FITC Antibody, clone 11-5B

Detect CNPase using this Milli-Mark Anti-CNPase-FITC Antibody, clone 11-5B validated for use in FC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neuronal & Glial Markers Gene Symbol: CNP, CNPase, CNP1, EC 3.1.4.37 UniProt Number: P09543
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FCMAB314F
100 tests  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMFITCMonoclonal Antibody
Description
Catalogue NumberFCMAB314F
Trade Name
  • Milli-Mark
DescriptionMilli-Mark Anti-CNPase-FITC Antibody, clone 11-5B
Alternate Names
  • 2'3'-Cyclic Nucleotide 3'-Phosphohydrolase
Background InformationCNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase [or -phosphohydrolase], EC 3.1.4.37) is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligo-dendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively.
Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.
References
Product Information
FormatFITC
Control
  • U251 Cells
PresentationPurified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationDetect CNPase using this Milli-Mark Anti-CNPase-FITC Antibody, clone 11-5B validated for use in FC.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenPurified human brain CNPase protein.
Clone11-5B
HostMouse
SpecificityAntibody recognizes Human CNPase protein.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CNP
  • CNPase
  • CNP1
  • EC 3.1.4.37
Purification MethodProtein A Purfied
UniProt Number
UniProt SummarySIZE: 421 amino acids; 47579 Da

SUBCELLULAR LOCATION: Membrane. Melanosome. Note=Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

SIMILARITY: SwissProt: P09543 ## Belongs to the cyclic nucleotide phosphodiesterase family.
Molecular Weight48kDa Calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by flow cytometry using U251 Cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
FCMAB314F 04053252754579

Documentation

Milli-Mark Anti-CNPase-FITC Antibody, clone 11-5B SDS

Title

Safety Data Sheet (SDS) 

Milli-Mark Anti-CNPase-FITC Antibody, clone 11-5B Certificates of Analysis

TitleLot Number
Milli-Mark Anti-CNPase-FITC, clone 11-5B - 2204987 2204987
Milli-Mark#174; Anti-CNPase-FITC, clone 11-5B - 2153058 2153058
Milli-Mark#174; Anti-CNPase-FITC, clone 11-5B - 2047696 2047696
Milli-Mark#174; Anti-CNPase-FITC, clone 11-5B - NRG1919865 NRG1919865
Milli-Mark™ Anti-CNPase-FITC, -2716777 2716777
Milli-Mark™ Anti-CNPase-FITC, -2820238 2820238
Milli-Mark™ Anti-CNPase-FITC, clone 11-5B - 3998961 3998961
Milli-Mark™ Anti-CNPase-FITC, clone 11-5B Conjugate 2937354
Milli-Mark™ Anti-CNPase-FITC, clone 11-5B Conjugate 2939556
Milli-Mark™ Anti-CNPase-FITC,clone 11-5B - 3150398 3150398
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