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FCMAB101A4 Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor® 488 conjugated

Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor 488 conjugated detects level of BrdU & has been published & validated for use in FC & IC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: BrdU
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FCMAB101A4
100 tests  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, ICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue NumberFCMAB101A4
Trade Name
  • Milli-Mark
DescriptionMilli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor® 488 conjugated
Alternate Names
  • BrdU
Background InformationBromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. This antibody does not require pretreatment of cells with strong DNA denaturants in order for the antibody to recognize incorporated BrdU. The antibody's special ability is thought to be due to the presence of DNAse in the antibody solution arising from the mycoplasma infecting the original hybridoma line {Gonchoroff, 1985}.
References
Product Information
FormatAlexaFluor®488
HS Code3002 15 90
Control
  • After incorporation of BrdU, all DNA containing species
PresentationPresentation needs to say: Purified mouse monoclonal IgG2a supernatant conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. Possible mycoplasma risk contained within this product. Use appropriate measures to prevent cell line infection/transmission.
Quality LevelMQ100
Applications
ApplicationMilli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor 488 conjugated detects level of BrdU & has been published & validated for use in FC & IC.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
Biological Information
ImmunogenBromodeoxyuridine
CloneBu-1
HostMouse
SpecificitySpecifically binds to bromodeoxyuridine (crossreacts with iodouridine 10%). Does not crossreact with fluorodeoxyuridine, nor with any endogenous cellular components such as thymidine or uridine.
IsotypeIgG2a
Species Reactivity
  • Human
Species Reactivity NoteTested on Human. Predicted to react with all species.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • BrdU
Purification MethodProtein G Purified
Molecular Weightnone given
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by flow cytometry with Jurkat Cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at 2-8°C from date of receipt. Protect from light.
Packaging Information
Material Size100 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
FCMAB101A4 04053252742194

Documentation

Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor® 488 conjugated SDS

Title

Safety Data Sheet (SDS) 

Milli-Mark Anti-BrdU Antibody, clone BU-1 Alexa Fluor® 488 conjugated Certificates of Analysis

TitleLot Number
Anti-BrdU, clone BU-1 Alexa Fluor#174; 488 conjugated - NG1741004 NG1741004
Anti-BrdU, clone BU-1 Alexa Fluor#174; 488 conjugated - NRG1678372 NRG1678372
Milli-Mark Anti-BrdU, clone BU-1 Alexa Fluor#174; 488 - 2024890 2024890
Milli-Mark Anti-BrdU, clone BU-1 Alexa Fluor#174; 488 - 2051959 2051959
Milli-Mark Anti-BrdU, clone BU-1 Alexa Fluor#174; 488 - 2154103 2154103
Milli-Mark Anti-BrdU, clone BU-1 Alexa Fluor#174; 488 - NG1818622 NG1818622
Milli-Mark Anti-BrdU, clone BU-1 Alexa Fluor#174; 488 - NG1827288 NG1827288
Milli-Mark Anti-BrdU, clone BU-1 Alexa Fluor#174; 488 - NG1837348 NG1837348
Milli-Mark Anti-BrdU, clone BU-1 Alexa Fluor#174; 488 - NG1842844 NG1842844
Milli-Mark Anti-BrdU, clone BU-1 Alexa Fluor#174; 488 - NG1928771 NG1928771
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Related Products & Applications

Alternative Format

Catalogue Number Description
MAB3510 Anti-BrdU Antibody, clone BU-1

Product Families

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Merck's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow.
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies