Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

371962 InSolution™ H-89, Dihydrochloride - Calbiochem

InSolution™ H-89, Dihydrochloride, CAS 127243-85-0, is a 10 mM solution of H-89, 2HCl in DMSO. A cell-permeable, potent, reversible, ATP-competitive inhibitor of protein kinase A (Ki = 48 nM).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: N-[2-((p-Bromocinnamyl)amino)ethyl]-5-isoquinolinesulfonamide, 2HCl, PKA Inhibitor III

Primary Target: PKA
View Products on Sigmaaldrich.com
371962
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Empirical Formula
C₂₀H₂₀BrN₃O₂S

Products

Catalogue NumberPackaging Qty/Pack
371962-1MG Plastic ampoule 1 mg
Description
Catalogue Number371962
Brand Family Calbiochem®
SynonymsN-[2-((p-Bromocinnamyl)amino)ethyl]-5-isoquinolinesulfonamide, 2HCl, PKA Inhibitor III
References
ReferencesLeemhuis, J., et al. 2002. J. Pharmacol. Exp. Ther. 300, 1000.
Davies, S.P. et al. 2000. Biochem. J. 351, 95.
de Rooij, J., et al. 1998. Nature. 396, 474.
Kawasaki, H., et al. 1998. Science. 282, 2275.
Findik, D., et al. 1995. J. Cell. Biochem. 57, 12.
Hidaka, H., and Kobayashi, R. 1992. Annu. Rev. Pharmacol. Toxicol. 32, 377.
Geilen, C.C., et al. 1992. FEBS Lett. 309, 381.
Chijiwa, T., et al. 1990. J. Biol. Chem. 265, 5267.
Combest, W.L., et al. 1988. J. Neurochem. 51, 1581.
Product Information
ATP CompetitiveN
FormLiquid
FormulationA 10 mM (1 mg/193 µl) solution of H-89, 2HCl (Cat. No. 371963) in DMSO.
Hill FormulaC₂₀H₂₀BrN₃O₂S
Chemical formulaC₂₀H₂₀BrN₃O₂S
ReversibleN
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationInSolution™ H-89, Dihydrochloride, CAS 127243-85-0, is a 10 mM solution of H-89, 2HCl in DMSO. A cell-permeable, potent, reversible, ATP-competitive inhibitor of protein kinase A (Ki = 48 nM).
Biological Information
Primary TargetPKA
Primary Target K<sub>i</sub>48 nM against protein kinase A
Purity≥99% by HPLC
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/38

Irritating to eyes and skin.
S PhraseS: 26

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Irritant
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
371962-1MG 04055977213515

Documentation

InSolution™ H-89, Dihydrochloride - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

InSolution™ H-89, Dihydrochloride - Calbiochem Certificates of Analysis

TitleLot Number
371962

References

Reference overview
Leemhuis, J., et al. 2002. J. Pharmacol. Exp. Ther. 300, 1000.
Davies, S.P. et al. 2000. Biochem. J. 351, 95.
de Rooij, J., et al. 1998. Nature. 396, 474.
Kawasaki, H., et al. 1998. Science. 282, 2275.
Findik, D., et al. 1995. J. Cell. Biochem. 57, 12.
Hidaka, H., and Kobayashi, R. 1992. Annu. Rev. Pharmacol. Toxicol. 32, 377.
Geilen, C.C., et al. 1992. FEBS Lett. 309, 381.
Chijiwa, T., et al. 1990. J. Biol. Chem. 265, 5267.
Combest, W.L., et al. 1988. J. Neurochem. 51, 1581.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-September-2007 JSW
SynonymsN-[2-((p-Bromocinnamyl)amino)ethyl]-5-isoquinolinesulfonamide, 2HCl, PKA Inhibitor III
DescriptionA solution of H-89, Dihydrochloride (Cat. No. 371963) in anhydrous DMSO. H-89 is a cell-permeable selective and potent inhibitor of protein kinase A (Ki = 48 nM). Inhibits other kinases at several fold higher concentrations: myosin light chain kinase (Ki = 28.3 µM), Ca2+/calmodulin-dependent protein kinase II (Ki = 29.7 µM), protein kinase C (Ki = 31.7 µM), casein kinase I (Ki = 38.3 µM), and Rho Kinase II (IC50 = 270 nM). May be used to discriminate between the effects of PKA and cAMP-regulated guanine-nucleotide-exchange factors (GEFs), such as GEFI or Epac (exchange protein directly activated by cAMP) and GEFII. Reported to induce neurite formation in NG 108-15 cells (~1 µM) by blocking the action of Rho kinase II.
FormLiquid
FormulationA 10 mM (1 mg/193 µl) solution of H-89, 2HCl (Cat. No. 371963) in DMSO.
Intert gas (Yes/No) Packaged under inert gas
Chemical formulaC₂₀H₂₀BrN₃O₂S
Structure formulaStructure formula
Purity≥99% by HPLC
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Irritant
ReferencesLeemhuis, J., et al. 2002. J. Pharmacol. Exp. Ther. 300, 1000.
Davies, S.P. et al. 2000. Biochem. J. 351, 95.
de Rooij, J., et al. 1998. Nature. 396, 474.
Kawasaki, H., et al. 1998. Science. 282, 2275.
Findik, D., et al. 1995. J. Cell. Biochem. 57, 12.
Hidaka, H., and Kobayashi, R. 1992. Annu. Rev. Pharmacol. Toxicol. 32, 377.
Geilen, C.C., et al. 1992. FEBS Lett. 309, 381.
Chijiwa, T., et al. 1990. J. Biol. Chem. 265, 5267.
Combest, W.L., et al. 1988. J. Neurochem. 51, 1581.