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371963 H-89, Dihydrochloride - CAS 127243-85-0 - Calbiochem

Overview

Key Spec Table

CAS #Empirical Formula
127243-85-0C₂₀H₂₀BrN₃O₂S · 2HCl

Products

Catalogue NumberPackaging Qty/Pack
371963-1MG Plastic ampoule 1 mg
Description
OverviewA cell-permeable, selective, reversible, ATP-competitive and potent inhibitor of protein kinase A (Ki = 48 nM). Inhibits other kinases only at much higher concentrations: CaM kinase II (Ki = 29.7 µM), casein kinase I (Ki = 38.3 µM), myosin light chain kinase (Ki = 28.3 µM), protein kinase C (Ki = 31.7 µM), and ROCK-II (IC50 = 270 nM). May be used to discriminate between the effects of PKA and cAMP-regulated guanine-nucleotide-exchange factors (GEFs) such as GEFI, GEFII, or Epac (exchange protein directly activated by cAMP). Enhances radiation-induced apoptosis in the human cell line BM 13674. A 10 mM (1 mg/193 µl) solution of H-89, 2HCl (Cat. No. 371962) in DMSO is also available.
Catalogue Number371963
Brand Family Calbiochem®
SynonymsN-[2-((p-Bromocinnamyl)amino)ethyl]-5-isoquinolinesulfonamide, 2HCl, PKA Inhibitor III
References
ReferencesLeemhuis, J., et al. 2002. J. Pharmacol. Exp. Ther. 300, 1000.
Davies, S.P., et al. 2000. Biochem. J. 351, 95.
Kawasaki, H., et al. 1998. Science 282, 2275.
de Rooij, J., et al. 1998. Nature 396, 474.
Findik, D., et al. 1995. J. Cell. Biochem. 57, 12.
Geilen, C.C. 1992. FEBS Lett. 309, 381.
Chijiwa, T., et al. 1990. J. Biol. Chem. 265, 5267.
Combest, W.L., et al. 1988. J. Neurochem. 51, 1581.
Product Information
CAS number127243-85-0
ATP CompetitiveY
FormClear to off-white crystalline film
Hill FormulaC₂₀H₂₀BrN₃O₂S · 2HCl
Chemical formulaC₂₀H₂₀BrN₃O₂S · 2HCl
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
ApplicationH-89, Dihydrochloride, CAS 127243-85-0, is a cell-permeable, potent, selective, reversible, ATP-competitive inhibitor of protein kinase A (Ki = 48 nM).
Biological Information
Primary TargetPKA
Primary Target K<sub>i</sub>48 nM for protein kinase A
Purity≥99% by HPLC
Physicochemical Information
Cell permeableY
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for at up to 4 months at 4°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Global Trade Item Number
Catalogue Number GTIN
371963-1MG 04055977213522