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345749 GDF-8 Propeptide, His•Tag®, Mouse, Recombinant

GDF-8 Propeptide, His•Tag®, Mouse, Recombinant, consists of amino acids 25-285, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Myostatin Propeptide

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345749
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Overview

Replacement Information

Key Spec Table

Purity
≥90% by SDS-PAGE

Products

Catalogue NumberPackaging Qty/Pack
345749-25UG Glass bottle 25 μg
Description
OverviewRecombinant, mouse growth differentiation factor-8 (GDF-8)/myostatin propeptide, consisting of amino acids 25-285, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. GDF-8 is a secreted protein that is expressed during embryonic development and in adult skeletal muscle.
Catalogue Number345749
Brand Family Calbiochem®
SynonymsMyostatin Propeptide
References
ReferencesHill, J.J. et al. 2003. Molecular Endocrinology 17, 1144.
Thies, R.S. et al. 2001. Growth Factors 18, 251.
Chang, H. et al. 2002. Endocrine Reviews 23, 787.
McPherron, A.C. et al. 1997. Nature 387, 83.
Product Information
FormLyophilized
FormulationLyophilized from 0.2 µm filtered PBS, BSA.
Quality LevelMQ100
Applications
ApplicationGDF-8 Propeptide, His•Tag®, Mouse, Recombinant, consists of amino acids 25-285, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells.
Biological Information
Biological activityED₅₀ = 0.05-0.25 µg/ml (using 40 ng/ml rmGDF-8) as measured by inhibition of rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Approximately 1 µg/ml will completely inhibit GDF-8 activity in K562 cells.
Purity≥90% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstituyion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
345749-25UG 04055977193893

Documentation

GDF-8 Propeptide, His•Tag®, Mouse, Recombinant SDS

Title

Safety Data Sheet (SDS) 

GDF-8 Propeptide, His•Tag®, Mouse, Recombinant Certificates of Analysis

TitleLot Number
345749

References

Reference overview
Hill, J.J. et al. 2003. Molecular Endocrinology 17, 1144.
Thies, R.S. et al. 2001. Growth Factors 18, 251.
Chang, H. et al. 2002. Endocrine Reviews 23, 787.
McPherron, A.C. et al. 1997. Nature 387, 83.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-June-2011 RFH
SynonymsMyostatin Propeptide
DescriptionRecombinant, mouse growth differentiation factor-8 (GDF-8)/myostatin propeptide, consisting of amino acids 25-285, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. GDF-8 is a secreted protein that is expressed during embryonic development and in adult skeletal muscle.
FormLyophilized
FormulationLyophilized from 0.2 µm filtered PBS, BSA.
Purity≥90% by SDS-PAGE
ContaminantsEndotoxin: ≤1.0 EU/µg protein
Biological activityED₅₀ = 0.05-0.25 µg/ml (using 40 ng/ml rmGDF-8) as measured by inhibition of rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Approximately 1 µg/ml will completely inhibit GDF-8 activity in K562 cells.
SolubilityReconstitute to 50 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstituyion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesHill, J.J. et al. 2003. Molecular Endocrinology 17, 1144.
Thies, R.S. et al. 2001. Growth Factors 18, 251.
Chang, H. et al. 2002. Endocrine Reviews 23, 787.
McPherron, A.C. et al. 1997. Nature 387, 83.