Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

GF144 Epidermal Growth Factor Protein, Human recombinant

The Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal & epithelial cells.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: P01133 Entrez Gene Number: NM_001963.2
View Products on Sigmaaldrich.com
GF144
500 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

SourcePurity
E.coliGreater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of EGF.
Description
Catalogue NumberGF144
ReplacesGF001
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionEpidermal Growth Factor Protein, Human recombinant
OverviewEpidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human EGF is a 6.2 kDa protein containing 53 amino acid residues.
Alternate Names
  • EGF
References
Product Information
HS Code2937 19 90
PresentationLyophilized from sterile filtered 10 mM Sodium Phosphate, pH 7.5, containing no additives.
Quality LevelMQ100
Applications
ApplicationThe Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal & epithelial cells.
Application NotesFor most in vitro applications, EGF exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL. Responding cells are (partial list): Epidermal mesothelial cells.
Biological Information
PurityGreater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of EGF.
SourceE.coli
Specific ActivityHuman EGF is fully biologically active when compared to standards. The ED50, as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells, is ≤ 0.1 ng/mL, corresponding to a specific activity of ≥ 1 x 10^7 units/mg.
Entrez Gene Number
Entrez Gene SummaryEpidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
Gene Symbol
  • EGF
  • URG
  • urogastrone
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01133 # EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
SIZE: 1207 amino acids; 133946 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P01133 ## Contains 9 EGF-like domains. & Contains 9 LDL-receptor class B repeats.
Stem Cell Type
  • Neural Stem Cells
  • Epithelial Cells
  • Mesenchymal Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe lyophilized EGF, though stable at room temperature, is best stored desiccated at -20°C. Reconstitute with water to a concentration of 0.1 - 1 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for 1 week or -20°C for future use. Reconstituted EGF should be maintained at -20°C in undiluted aliquots for up to one year.
Packaging Information
Material Size500 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF144 08436037123825

Documentation

Epidermal Growth Factor Protein, Human recombinant SDS

Title

Safety Data Sheet (SDS) 

Epidermal Growth Factor Protein, Human recombinant Certificates of Analysis

TitleLot Number
EGF, HUMAN RECOMBINANT PROTEIN 2934694
EGF, HUMAN RECOMBINANT PROTEIN 2845216
EGF, HUMAN RECOMBINANT PROTEIN 2919892
EGF, HUMAN RECOMBINANT PROTEIN - 2135704 2135704
EGF, HUMAN RECOMBINANT PROTEIN - 2384721 2384721
EGF, HUMAN RECOMBINANT PROTEIN - 2444147 2444147
EGF, HUMAN RECOMBINANT PROTEIN - 1988719 1988719
EGF, HUMAN RECOMBINANT PROTEIN - 2052446 2052446
EGF, HUMAN RECOMBINANT PROTEIN - 2066038 2066038
EGF, HUMAN RECOMBINANT PROTEIN - 2073747 2073747
Show more

Data Sheet

Title
EGF, HUMAN RECOMBINANT PROTEIN

Newsletters / Publications

Title
Cellutions Newsletter: 2007, Volume 1
Cellutions Newsletter: 2008, Volume 1
Cellutions Newsletter: 2008, Volume 3

Related Products & Applications

Product Families

Cytokines and Growth Factors

EMD Millipore offers a comprehensive range of highly purified bioactive cytokines and growth factors for use in human, mouse, and rat cell culture, stem cell differentiation and ELISA studies.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors