Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

SCM082 ES2N Complete Medium Kit

View Products on Sigmaaldrich.com
SCM082
250 mL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
Cult
Description
Catalogue NumberSCM082
DescriptionES2N Complete Medium Kit
OverviewES2N Complete medium is a defined serum-free formulation that has been optimized for the differentiation of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells into neurons. When used in conjucture with ESGRO Complete Plus medium mouse ES and iPS cells readily differentiate into neurons in a monolayer assay within 9-12 days on gelatin coated culture dishes. When supplemented with bFGF and EGF, this medium enables the isolation and expansion of neural stem cells (NSCs).
References
Product Information
Components
  • ES2N Basal Medium: (Cat. No. SCM083) One (1) 245 mL bottle containing standard base medium.
  • Neuro27 Medium Supplement: (Cat. No. SCM013-S) One (1) 5 mL vial containing neural supplement (use as 50x).
  • Neuro2 Medium Supplement: (Cat. No. SCM012-S) One (1) 1.25 mL vial containing N2-like supplement (use as 200x).
Quality LevelMQ300
Applications
ApplicationThe ES2N Complete medium is a defined serum-free formulation that has been optimized for the differentiation of mouse embryonic stem (ES) & induced pluripotent stem (iPS) cells into neurons.
Key Applications
  • Stem Cell Culture
Biological Information
Media FormLiquid
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • Induced Pluripotent Stem Cells
  • Neural Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceCell Proliferation Assay: The biological activity of this lot of VEGF was determined by its mitogenic activity on human umbilical vein endothelial cells (HUVEC) with an ED50 of about 1.0 ng/mL. Results may vary depending on the cell line used. VEGF will also stimulate endothelial cells from other species.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size250 mL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCM082 04053252699887

Documentation

ES2N Complete Medium Kit SDS

Title

Safety Data Sheet (SDS) 

ES2N Complete Medium Kit Certificates of Analysis

TitleLot Number
ES2N COMPLETE MEDIUM KIT 2968299
ES2N COMPLETE MEDIUM KIT 2914474
ES2N COMPLETE MEDIUM KIT 3021890
ES2N COMPLETE MEDIUM KIT 2969503
ES2N COMPLETE MEDIUM KIT - 2093596 2093596
ES2N COMPLETE MEDIUM KIT - 2358899 2358899
ES2N COMPLETE MEDIUM KIT - 2426531 2426531
ES2N COMPLETE MEDIUM KIT - 1997717 1997717
ES2N COMPLETE MEDIUM KIT - 2201822 2201822
ES2N COMPLETE MEDIUM KIT - 2300050 2300050

Brochure

Title
Murine Embryonic Stem Cell Culture Procedures & Protocols
Neuroscience Product Selection Guide

Data Sheet

Title
ES2N COMPLETE MEDIUM KIT
STEMCCA Lentivirus Reprogramming Kits