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GF170 DKK-1 Protein, Human Recombinant

The Dickkopf related protein 1 (or Dkk1 protein) is the founding member of the Dickkopf family of proteins that includes Dkk1, 2, 3, 4 & a related protein, Soggy.

DKK-1 Protein, Human Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: O94907
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GF170
10 µg  
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Overview

Replacement Information

Key Spec Table

SourcePurity
HEK293 CellsProduct is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 97% pure by SDS-PAGE gel and HPLC analyses
Description
Catalogue NumberGF170
DescriptionDKK-1 Protein, Human Recombinant
Alternate Names
  • Dickkopf related protein 1
  • DKK1
  • DKK
  • SK
Background InformationDickkopf related protein 1 (Dkk1) is the founding member of the Dickkopf family of proteins that includes Dkk1, 2, 3, 4, and a related protein, Soggy. Subsequent studies have shown that DKK-1 and DKK-4 play an important regulatory role in the Wnt /β-catenin signaling pathway by forming inhibitory complexes with LDL receptor-related proteins 5 and 6 (LRP5 and LRP6), which are essential components of the Wnt/βcatenin signaling system. LPR5 and LPR6 are single-pass transmembrane proteins that appear to act as co-receptors for Wnt ligands involved in the Wnt/βcatenin signaling cascade. It has been suggested that by inhibiting Wnt/β-catenin signaling, which is essential for posterior patterning in vertebrates, DKK-1 permits anterior development. This notion is supported by the finding that mice deficient of DKK-1 expression lack head formation and die during embryogenesis. Recombinant human DKK-1 expressed in human 293 cells is a 35-40 kDa glycoprotein containing 235 amino-acid residues.
References
Product Information
PresentationProduct is filtered through a 0.2 micron filter before lyophilization.
Quality LevelMQ100
Applications
ApplicationThe Dickkopf related protein 1 (or Dkk1 protein) is the founding member of the Dickkopf family of proteins that includes Dkk1, 2, 3, 4 & a related protein, Soggy.
Biological Information
PurityProduct is endotoxin free (< 0.1ng/μg of protein (<1EU/μg) and greater than 97% pure by SDS-PAGE gel and HPLC analyses
SourceHEK293 Cells
Cross ReactivityNone
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceBioactivity assay: Determined by its ability to inhibit the proliferation of HCT116 colorectal carcinoma cells. Approximately 40% growth inhibition was achieved at a DKK-1 concentration of 200ng/ml.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20°C for up to 6 months from date of receipt.
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF170 04053252691355

Documentation

DKK-1 Protein, Human Recombinant SDS

Title

Safety Data Sheet (SDS) 

DKK-1 Protein, Human Recombinant Certificates of Analysis

TitleLot Number
Recombinant Human -2573001 2573001
Recombinant Human -2604334 2604334
Recombinant Human DKK-1 2458038
Recombinant Human DKK-1 - 2039816 2039816
Recombinant Human DKK-1 - 2092740 2092740
Recombinant Human DKK-1 - 2219399 2219399
Recombinant Human DKK-1 - 2314807 2314807
Recombinant Human DKK-1 - 3068557 3068557
Recombinant Human DKK-1 - 3428775 3428775
Recombinant Human DKK-1 - 3552759 3552759
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Brochure

Title
New Products: Volume 3, 2012

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