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234409 Complement C9-Depleted Serum, Human

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234409
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Overview

Products

Catalogue NumberPackaging Qty/Pack
234409-1ML Plastic ampoule 1 ml
Description
OverviewHuman serum depleted of complement component C9 by affinity chromatography.
Catalogue Number234409
Brand Family Calbiochem®
Product Information
Unit of Definition<b>Classical Pathway Activity:</b> One C9H50 unit measured by classical pathway activation is defined as the amount of C9 required to yield 50% lysis of 3 x 10⁷EA when incubated in the presence of the recommended volume of C9-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++. <b>CH50 Functional Activity upon Reconstitution:</b> One CH50 unit is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1 x 10⁸ EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++. <b>Alternative Pathway Activity:</b> One unit of whole alternative pathway activity (APH50) is defined as the input of C9-Dpl, reconstituted with C9, or NHS Complement Standard yielding 50% lysis of 1.5 x 10⁷ rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 µl GVB° containing a final Mg-EGTA concentration of 13.3 mM.
FormLiquid
FormulationIn 150 mM NaCl, 50 mM sodium phosphate, pH 7.2 ± 0.2.
PreservativeNone
Biological Information
SourcePrepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Global Trade Item Number
Catalogue Number GTIN
234409-1ML 04055977200225