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227036 Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free - CAS 9012-63-9 - Calbiochem

Cholera toxin Inaba 569B, CAS 9012-63-9, has a single A subunit surrounded by five polypeptides chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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227036
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Overview

Replacement Information

Key Spec Table

CAS #
9012-63-9

Products

Catalogue NumberPackaging Qty/Pack
US1227036-1MG Glass bottle 1 mg
Description
OverviewUseful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface.
Useful for tissue culture applications.
Catalogue Number227036
Brand Family Calbiochem®
References
ReferencesGilman, A.G. 1987. Annu. Rev. Biochem. 56, 615.
Product Information
CAS number9012-63-9
ActivityBinding activity is assessed by hemagglutination assay using trisialoganglioside fixed sheep red blood cells.
ATP CompetitiveN
DeclarationNot available for sale outside of the United States.
FormSolid
FormulationLyophilized from 200 mM NaCl, 50 mM Tris, 1 mM EDTA, pH 7.5.
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary Targetα-subunit of G-proteins
Purity≥95% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSLF3100000
Safety Information
R PhraseR: 21/22-36/37/38

Harmful in contact with skin and if swallowed.
Irritating to eyes, respiratory system and skin.
S PhraseS: 7-36/37

Keep container tightly closed.
Wear suitable protective clothing and gloves.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Highly Toxic
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Do not freeze Yes
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 6 months at 4°C under sterile conditions.
End use certificateY
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
US1227036-1MG 04055977200560

Documentation

Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free - CAS 9012-63-9 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Cholera Toxin, Vibrio cholerae, Type Inaba 569B, Azide Free - CAS 9012-63-9 - Calbiochem Certificates of Analysis

TitleLot Number
227036

References

Reference overview
Gilman, A.G. 1987. Annu. Rev. Biochem. 56, 615.

Brochure

Title
Activators and Inhibitors of Adenylate Cyclase Technical Bulletin
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-March-2024 JSW
DescriptionUseful for studies involving adenylate cyclase, AMP, and related membrane-transport phenomena. Consists of a single A subunit surrounded by five polypeptide chains of the B subunits. The A subunit catalyzes the ADP-ribosylation of the α-subunit of G-proteins, reducing intrinsic GTPase activity and activating the α-subunit. The B subunits are responsible for the attachment of the native toxin on the cell surface..
FormSolid
FormulationLyophilized from 200 mM NaCl, 50 mM Tris, 1 mM EDTA, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
CAS number9012-63-9
RTECSLF3100000
Purity≥95% by SDS-PAGE
ActivityBinding activity is assessed by hemagglutination assay using trisialoganglioside fixed sheep red blood cells.
SolubilityPlease refer to the vial label for lot-specific reconstitution. Note: cholera toxin tends to breakdown into its A and B subunits when the solution is frozen or with vigorous shaking during reconstitution, resulting in damage to the toxin protein and concomitant loss of biological activity. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that would lead to foaming. Heating of cholera toxin in excess of 40-50°C results in aggregation and subsequent breakdown into its A and B subunits.
Storage +2°C to +8°C
Do Not Freeze Yes
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Aqueous stock solutions are stable for up to 6 months at 4°C under sterile conditions.
Toxicity Highly Toxic
ReferencesGilman, A.G. 1987. Annu. Rev. Biochem. 56, 615.