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23-053M CHIP (c-Myc tagged) Protein, active, 250 µg

Active, full length, recombinant human CHIP. N-terminal c-Myc, 6His-tagged. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.

CHIP (c-Myc tagged) Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
23-053M
250 µg  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Key ApplicationsSpeciesCascade Component
Enzyme AssaysHuman E3
Description
Catalogue Number23-053M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCHIP (c-Myc tagged) Protein, active, 250 µg
Alternate Names
  • Carboxy terminus of Hsp70-interacting protein
  • CLL-associated antigen KW-8
  • Antigen NY-CO-7
  • STIP1 homology and U box-containing protein 1
  • STUB1
Background InformationCHIP is an E3 ubiquitin-protein ligase which targets misfolded chaperone substrates for proteasomal degradation. It contains multiple domains, including a tetratricopeptide repeat (TPR) domain that allows interaction with molecular chaperones, such as Hsp70 and Hsp90 and a U-box domain that confers its E3 ubiquitin ligase function. It has been demonstrated that CHIP preferentially ubiquitinates chaperone-bound substrates, of which there are a diverse range including CFTR, ErbB2 and LRRK2, but also targets Hsp70 itself to the proteasome for degradation when misfolded substrates are not present. This sequential degradation of misfolded substrate and chaperone provides a homeostatic mechanism for regulating chaperone levels to reflect the status of protein folding within the cytoplasm.
Product Information
Quality LevelMQ300
Applications
ApplicationActive, full length, recombinant human CHIP. N-terminal c-Myc, 6His-tagged. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
Key Applications
  • Enzyme Assays
Biological Information
Cascade ComponentE3
Entrez Gene Number
Purification MethodPurified using immobilized metal affinity chromatography.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Packaging Information
Material Size250 µg
Global Trade Item Number
Catalogue Number GTIN
23-053M 04053252599750