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SCT276 BioTracker™ Green pH-Independent Autophagy Live Cell Probe

Fluorescence microscopy
Flow cytometry

SCT276
5 nmol  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberSCT276
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DescriptionBioTracker™ Green pH-Independent Autophagy Live Cell Probe
OverviewSCT276 is a small fluorescent molecule that detects autophagosomes and autolysosomes by a likely mechanism of incorporation into the autophagosome double membrane during its formation, whereupon it emits fluorescence under hydrophobic conditions. This probe has been noted to frequently colocalize with LC3.

SPECTRAL CHARACTERISTICS:
Excitation λ: 450 nm
Emission λ: 535 nm
Alternate Names
  • green autophagy probe
  • autophagosome live cell probe
  • green autophagosome probe
  • green AP live cell probe
Background InformationAutophagy is an intracellular degradation system, where dysfunctional proteins and organelles are degraded. In this process, dysfunctional proteins aggregate and are enclosed by a double membrane to form an autophagosome.
Product Information
Detection methodFlow cytometry Fluorescence
FormatLyophilized solid
PresentationLyophilized solid.
Quality LevelMQ100
Applications
ApplicationFluorescence microscopy
Flow cytometry
Key Applications
  • Cell Based Assays
  • Fluorescence
  • Flow Cytometry
  • Fluorescence Activated Cell Sorting (FACS)
Application NotesSCT276 detects autophagosomes and autolysosomes by the likely mechanism of incorporation into the autophagosome during double membrane formation, and emits green fluorescence under hydrophobic conditions. SCT276 is cell permeable, does not require transfection, and enables live cell imaging with fluorescence microscopy and quantitative assay by flow cytometry.


Add 50 uL DMSO to tube of SCT276 (5 nmol) and pipet to dissolve to make a 0.1 mM stock solution. Dilute with culture medium to 0.1 - 0.5 uM to make working solution.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20 °C, protected from light.
Packaging Information
Material Size5 nmol
Global Trade Item Number
Catalogue Number GTIN
SCT276 04065269218562