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SCT271 BioTracker™ Green Endocytosis Live Cell Probe

pH-dependent fluorescent live cell probe for visualization of endocytosis from the early endosome stage.
Fluorescent Dye-Dextran Conjugates or membrane staining reagents are used to visualize endocytosis. However, they have limitations in observing the dynamics of endosomes in live cells in terms of precision of staining or retentivity of reagent. SCT271 is a different kind of reagent that overcomes these limitations.

NewSCT271
40 µL  
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Overview

Replacement Information
Description
Catalogue NumberSCT271
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DescriptionBioTracker™ Green Endocytosis Live Cell Probe
OverviewBioTracker™ Green Endocytosis Live Cell Probe is a pH-dependent fluorescent dye that localizes to the vesicle membrane. Visualization of endocytosis using SCT271 is a more direct method than fluorescent analogs, and allows visualization of endocytosis.

*Precise visualization of endocytosis
*Track endocytosis using live cells
*High responsiveness to pH change


SPECTRAL PROPERTIES
λex: 386 nm
λem: 522 nm

Suggested observation conditions:
λex: 350 - 410 nm
λem: 500 - 560 nm
Alternate Names
  • endocytosis probe
  • green endocytosis probe
  • endocytosis live cell probe
  • endocytosis fluorescent probe
Background InformationEndocytosis is a cellular process that describes macromolecules being taken up through the plasma membrane-derived endosome vesicle. The endocytic pathway contributes to maintaining intracellular homeostasis by transporting various nutrients into the cell and shuttling unneeded components to the lysosome, which functions as a waste disposal system. It has been demonstrated that disruption of endocytosis is related to some neurodegenerative dysfunction and immune diseases. Investigation of the endocytic pathway is therefore of increasing interest to cell biologists and in diverse disease disciplines.
References
Product Information
FormatLiquid Reagent
Presentationgreen liquid
Quality LevelMQ100
Applications
ApplicationpH-dependent fluorescent live cell probe for visualization of endocytosis from the early endosome stage.
Fluorescent Dye-Dextran Conjugates or membrane staining reagents are used to visualize endocytosis. However, they have limitations in observing the dynamics of endosomes in live cells in terms of precision of staining or retentivity of reagent. SCT271 is a different kind of reagent that overcomes these limitations.
Key Applications
  • Cell Based Assays
  • Fluorescence
Application NotesThis live cell probe is a non membrane-permeable molecule that fluoresces on internalization into mammalian cells,enabling direct observation of the membrane of internalized vesicles.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -20 °C, protected from light.
Packaging Information
Material Size40 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCT271 04065270661326