Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

SCT069 BioTracker ATP-LW Live Cell Dye

Live cell imaging dye for simultaneous visualization of cellular Adenosine-5'-Triphosphate (ATP) and peroxynitrite (ONOO-).

View Products on Sigmaaldrich.com
SCT069
1 mg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCT069
DescriptionBioTracker ATP-LW Live Cell Dye
OverviewAdenosine-5’-triphosphate (ATP) plays an vital role in regulating cell movement, neurotransmission, and ion channels. Disruption of ATP homeostasis can induce diseases including ischemia, Parkinson’s disease and hypoglycaemia.

Peroxynitrite (ONOO-) is a highly reactive nitrogen species with an extremely short biological half-life. ONOO- is known for its deleterious effects, causing irreversible damage to a range of biological targets such as lipids, proteins and nucleic acids. As a result, ONOO- is associated with diseases such as inflammation, cancer, atherosclerosis, ischemia-reperfusion injury and neurodegenerative diseases.

BioTracker ATP-LW live cell dye is a dual probe for detection and imaging of ATP and peroxynitrite in live cells. The dye did not respond to presence of other reactive nitrogen/oxygen species such as H2O2 and HClO. However, ADP (adenosine diphosphate) and CTP (cytosine-5'-triphosphate) induced fluorescence enhancements.

Note: BioTracker ATP-LW live cell dye is stable in DMSO but unstable is water and aqueous solution. Prepare the working solution and use it immediately.

Spectral Properties
For ATP detection: Excitation: 450 nm
Emission: 550 nm

For Peroxynitrite detection: Excitation: 520 nm
Emission: 586 nm

References
Wu L et al. Dual-function fluorescent probe for the detection of peroxynitrite and adenosine triphosphate. ChemRvix, 27 Feb 2019.
Alternate Names
  • Live cell imaging probe
References
Product Information
Detection methodFluorescence
PresentationLyophilized
Applications
ApplicationLive cell imaging dye for simultaneous visualization of cellular Adenosine-5'-Triphosphate (ATP) and peroxynitrite (ONOO-).
Key Applications
  • Cell Based Assays
Application NotesLive cell fluorescent imaging
Biological Information
ConcentrationPlease refer to lot specific datasheet.
Molecular Weight833.82 g/mol
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceConfirmed by HNMR, LC-MS and elemental analysis
Molar Mass: 883.82 g/mol. BioTracker ATP-LW live cell dye is stable in DMSO but unstable is water and aqueous solution. Prepare the working solution and use it immediately.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker ATP-LW Live Cell Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCT069 04061841078111

Documentation