Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

616371 Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free - CAS 9087-70-1 - Calbiochem

Aprotinin, Bovine, Recombinant, Nicotiana sp, Animal-Free - CAS 9087-70-1, is a heat and acid-stable competitive, reversible inhibitor of serine proteases.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Pancreatic Trypsin Inhibitor, Trypsin-Kallikrein Inhibitor, Kallikrein-Trypsin Inactivator, Antikrein, Basic Pancreatic Trypsin Inhibitor

Primary Target: chymotrypsin
View Products on Sigmaaldrich.com
616371
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
9087-70-1C₂₈₄H₄₃₂N₈₄O₇₉S₇

Products

Catalogue NumberPackaging Qty/Pack
616371-1MG Plastic ampoule 1 mg
616371-25MG Glass bottle 25 mg
616371-5MG Plastic ampoule 5 mg
Description
OverviewRecombinant, bovine aprotinin supplied without animal-derived components. Aprotinin is a competitive, reversible inhibitor of proteolytic and esterolytic activity. A relatively heat- and acid-stable serine protease inhibitor. Forms a tight complex, blocking the active site of the enzyme. Effective at concentrations equimolar with protease. Inhibits several proteases, including coagulation factors in the prephase of blood clotting, tissue and leukocytic proteinases, chymotrypsin, trypsin (Kd = 5 x 10-14 M), plasmin (Kd = 2.3 x 10-10 M), and kallikrein (Kd = 1 x 10-7 M). Proteases not inhibited by aprotinin include Factor Xa, thrombin, pepsin, papain, and carboxypeptidases A and B. Useful for protein purification and for extending the life of cells in culture by preventing proteolytic damage.
Catalogue Number616371
Brand Family Calbiochem®
SynonymsPancreatic Trypsin Inhibitor, Trypsin-Kallikrein Inhibitor, Kallikrein-Trypsin Inactivator, Antikrein, Basic Pancreatic Trypsin Inhibitor
References
ReferencesDeutscher, M.P., 1990. Methods Enzymol. 182, 83.
Product Information
CAS number9087-70-1
Unit of DefinitionOne TIU (Trypsin Inhibitory Unit) will decrease the activity of two trypsin units by 50% where one trypsin unit will hydrolyze 1.0 µmole of Nα-benzoyl-L-Arginine-p-Nitroanilide (L-BAPNA) per minute at pH 7.8 at 25°C.
ATP CompetitiveN
EC number2329949
FormWhite to off-white powder
FormulationContains no animal-derived components.
Hill FormulaC₂₈₄H₄₃₂N₈₄O₇₉S₇
Chemical formulaC₂₈₄H₄₃₂N₈₄O₇₉S₇
ReversibleY
Quality LevelMQ200
Applications
Biological Information
Primary Targetchymotrypsin
Primary Target IC<sub>50</sub>Kd = 5 x 10-14 M, 2.3 x 10-10 M, 1 x 10-7 M against trypsin, plasmin, and kallikrein, respectively
Purity≥98% by SDS-PAGE
Specific Activity≥5.0 EPU/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSYN5080000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
616371-1MG 04055977262919
616371-25MG 04055977262926
616371-5MG 04055977262933

Documentation

Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free - CAS 9087-70-1 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free - CAS 9087-70-1 - Calbiochem Certificates of Analysis

TitleLot Number
616371

References

Reference overview
Deutscher, M.P., 1990. Methods Enzymol. 182, 83.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-August-2024 JSW
SynonymsPancreatic Trypsin Inhibitor, Trypsin-Kallikrein Inhibitor, Kallikrein-Trypsin Inactivator, Antikrein, Basic Pancreatic Trypsin Inhibitor
DescriptionRecombinant, bovine aprotinin supplied without animal-derived components. Aprotinin is a competitive, reversible inhibitor of proteolytic and esterolytic activity. A relatively heat- and acid-stable serine protease inhibitor. Forms a tight complex, blocking the active site of the enzyme. Effective at concentrations equimolar with protease. Inhibits several proteases, including coagulation factors in the prephase of blood clotting, tissue and leukocytic proteinases, chymotrypsin, trypsin (Kd = 5 x 10-14 M), plasmin (Kd = 2.3 x 10-10 M), and kallikrein (Kd = 1 x 10-7 M). Proteases not inhibited by aprotinin include Factor Xa, thrombin, pepsin, papain, and carboxypeptidases A and B. Useful for protein purification and for extending the life of cells in culture by preventing proteolytic damage.
FormWhite to off-white powder
FormulationContains no animal-derived components.
Concentration Label Please refer to vial label for lot-specific concentration
CAS number9087-70-1
RTECSYN5080000
EC number2329949
Chemical formulaC₂₈₄H₄₃₂N₈₄O₇₉S₇
Purity≥98% by SDS-PAGE
Specific activity≥5.0 EPU/mg protein
Unit definitionOne TIU (Trypsin Inhibitory Unit) will decrease the activity of two trypsin units by 50% where one trypsin unit will hydrolyze 1.0 µmole of Nα-benzoyl-L-Arginine-p-Nitroanilide (L-BAPNA) per minute at pH 7.8 at 25°C.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Toxicity Standard Handling
Merck USA index14, 757
ReferencesDeutscher, M.P., 1990. Methods Enzymol. 182, 83.