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07-357 Anti-phospho-eNOS/NOS III (Ser114) Antibody

Detect phospho-eNOS/NOS III (Ser114) using this Anti-phospho-eNOS/NOS III (Ser114) Antibody validated for use in WB.

Anti-phospho-eNOS/NOS III (Ser114) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Oxidative Stress Gene Symbol: NOS3, cNOS, ECNOS, eNOS, EC-NOS, NOSIII UniProt Number: P29474
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07-357
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-357
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-eNOS/NOS III (Ser114) Antibody
OverviewNote: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS
References
Product Information
FormatAffinity Purified
Presentation0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA, and 0.05% sodium azide before the addition of glycerol to 30%
Quality LevelMQ100
Applications
ApplicationDetect phospho-eNOS/NOS III (Ser114) using this Anti-phospho-eNOS/NOS III (Ser114) Antibody validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenSynthetic peptide corresponding to amino acids 106-118 of human eNOS/NOS III (Ser114) (PRKLQGRPpSPGPP-C) with a C-terminal cysteine added for conjugation to KLH. The immunizing sequence shares 12/13 amino acids with pig and bovine eNOS/NOS III.
HostRabbit
SpecificityeNOS/NOS III phosphorylated on serine 114
IsotypeIgG
Species Reactivity
  • Bovine
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NOS3
  • cNOS
  • ECNOS
  • eNOS
  • EC-NOS
  • NOSIII
Modifications
  • Phosphorylation
Purification MethodImmunoaffinity Chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
SIZE: 1203 amino acids; 133289 Da
SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN.
SUBCELLULAR LOCATION: Cell membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
TISSUE SPECIFICITY: Platelets, placenta, liver and kidney.
SIMILARITY: SwissProt: P29474 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
Molecular Weight132 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot in fetal bovine heart endothelial cell lysate
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -20°C
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-357 04053252404016

Documentation

Anti-phospho-eNOS/NOS III (Ser114) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-eNOS/NOS III (Ser114) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho-eNOS/NOS III (Ser114) (rabbit immunoaffinity purified IgG) 2973090
Anti-phospho-eNOS/NOS III (Ser114) - 3469483 3469483
Anti-phospho-eNOS/NOS III (Ser114) -2787698A 2787698A
Anti-phospho-eNOS/NOS III (Ser114) -2852433 2852433
Anti-phospho-eNOS/NOS III (Ser116) (rabbit immunoaffinity purified IgG) - 2149489 2149489
Anti-phospho-eNOS/NOS III (Ser116) (rabbit immunoaffinity purified IgG) - 2424503 2424503
Anti-phospho-eNOS/NOS III (Ser116) (rabbit immunoaffinity purified IgG) - 2273974 2273974
Anti-phospho-eNOS/NOS III (Ser116) - 22407 22407
Anti-phospho-eNOS/NOS III (Ser116) - 28830 28830
Anti-phospho-eNOS/NOS III (Ser116) -2604783 2604783
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References

Reference overviewPub Med ID
Functional protein network activation mapping reveals new potential molecular drug targets for poor prognosis pediatric BCP-ALL.
Accordi, Benedetta, et al.
PLoS ONE, 5: e13552 (2010)  2010

Show Abstract 		21042412 21042412

Brochure

Title
Pathways and Biomarkers of Oxidative Stress

Technical Info

Title
White Paper- Modern Methods in Oxidative Stress Research