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ABC1473-100UL Anti-phospho-c-Myc (Ser348)

Anti-phospho-c-Myc (Ser348), Cat. No. ABC1473, is a rabbit polyclonal antibody that detects c-Myc phosphorylated on serine 348 and is tested for use in Function Analysis, Peptide Inhibition Assay and Western Blotting.

ABC1473-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABC1473-100UL
DescriptionAnti-phospho-c-Myc (Ser348)
Alternate Names
  • Myc proto-oncogene protein
  • Class E basic helix-loop-helix protein 39
  • bHLHe39
  • Proto-oncogene c-Myc
  • c-Myc
  • Transcription factor p64
Background InformationMyc proto-oncogene protein (UniProt: P01106; also known as Class E basic helix-loop-helix protein 39, bHLHe39, Proto-oncogene c-Myc, Transcription factor p64) is encoded by the MYC (also known as BHLHE39) gene (Gene ID: 4609) in human. c-Myc is a transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. It regulates the expression of multiple genes involved in apoptosis, cell differentiation and cell proliferation. c-Myc is shown to be critically involved in the regulation of several growth promoting signaling pathways and is considered as an immediate early response gene that is downstream of many ligand-membrane receptor complexes. Its expression is reported to be tightly regulated by several transcriptional regulatory motifs within its proximal promoter region. Phosphorylation of c-Myc by serine/threonine kinase PIM2 at serine 329 is reported to lead to its stabilization. Active c-Myc is coupled to the MAX protein via its C-terminal helix-loop-helix leucine zipper (bHLHLZ) domain (aa 413-434) and this heterodimer interacts with a number of other transcription-related proteins. In normal cells its expression and function are regulated by developmental or mitogenic signals. c-Myc mRNA has a very short half-life and in the absence of positive regulatory signals there is a significant reduction in its transcription leading to low c-Myc protein levels that does not have any proliferative drive. However, in tumor cells its levels are increased either via mutations or induction via upstream oncogenic pathways. Serine 347/348 are shown to be phosphorylated by casein kinase II. S347A/S348A reported to be more potent oncogenic mutants compared with wild-type c-Myc. Mutations leading to S347A/S348A are known to enhance transformation of cells compared to wild types. Mutations in c-Myc gene have been linked to B-cell chronic lymphocytic leukemia and Burkitt lymphoma. Over-expression of c-Myc has been reported in about 40% tumors. (Ref.: Wasylishen, AR., et al. (2013). Cancer Res. 73(21); 6504-6515; Miller, DM et al., (2012). Clin. Cancer Res. 18(20); 5546 5553; Lutterbach, B., and Hann, SR (1997). Oncogene. 14(8); 967-975).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-phospho-c-Myc (Ser348), Cat. No. ABC1473, is a rabbit polyclonal antibody that detects c-Myc phosphorylated on serine 348 and is tested for use in Function Analysis, Peptide Inhibition Assay and Western Blotting.
Key Applications
  • Affects Function
  • Peptide Inhibition Assay
  • Western Blotting
Application NotesFunction Analysis: A representative lot of this antibody was used to detect the expression of gain of function with MYC mutants causing disruption of the normal morphogenesis in cells in 3D culture. (Courtesy of Dr. Linda Penn Lab @ Princess Margaret Cancer Centre, part of the University Health Network, Toronto).


Western Blotting Analysis: A 1: 500 dilution from a representative lot detected phospho-c-Myc (Ser348) in lysate from HEK293Tv cells transfected with V5-WT-Myc and treated with MG132 (Courtesy of Dr. Linda Penn Lab @ Princess Margaret Cancer Centre, part of the University Health Network, Toronto).

Peptide Inhibition Assay: A 1:500 dilution from a representative lot was used with HEK293Tv cells transfected with V5-WTMyc and treated with MG132 for peptide block analysis (Courtesy of Dr. Linda Penn Lab @ Princess Margaret Cancer Centre, part of the University Health Network, Toronto).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids from surrounding phosphoserine 348 from the C-terminal half of human c-Myc.
EpitopeC-terminus
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects c-Myc phosphorylated on serine 348. It targets an epitope within 10 amino acids surrounding phosphoserine 348 from the C-terminal half.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react non-Human Primates based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MYC
  • BHLHE39
Modifications
  • Phosphorylation
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~58 kDa observed; 48.80 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from HEK293Tv cells transfected with V5-WT-Myc.

Western Blotting Analysis: A 1:500 dilution of this antibody detected c-Myc proto-oncogene protein phosphorylated on serine 348 in lysate from HEK293T cells transfected with V5 tagged-wild-type c-Myc and treated with MG132 (10 µM, 4 h).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC1473-100UL 04061842667581