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ABC225 Anti-phospho USP7 (Ser18) Antibody

This Anti-phospho USP7 (Ser18) antibody is validated for use in western blotting & peptide inhibition assay for the detection of phospho USP7 (Ser18).

Anti-phospho USP7 (Ser18) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: USP7, HAUSP UniProt Number: Q93009
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ABC225
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, PIARbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABC225
DescriptionAnti-phospho USP7 (Ser18) Antibody
Alternate Names
  • Ubiquitin carboxyl-terminal hydrolase 7
  • Deubiquitinating enzyme 7
  • Herpesvirus-associated ubiquitin-specific protease
  • Ubiquitin thioesterase 7
  • Ubiquitin-specific-processing protease 7
Background InformationUbiquitin-specific-processing protease 7 (USP7) is also known as Ubiquitin carboxyl-terminal hydrolase 7 or Herpesvirus-associated ubiquitin-specific protease (HAUSP). USP7 is a ubiquitin specific protease or a deubiquitylating enzyme that cleaves ubiquitin from its substrates. Since ubiquitylation (polyubiquitination) is most commonly associated with the stability and degradation of cellular proteins, USP7 activity generally stabilizes its substrate proteins, and it is most popularly known as a direct antagonist of Mdm2, the E3 ubiquitin ligase for the tumor suppressor protein, p53.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho USP7 (Ser18) antibody is validated for use in western blotting & peptide inhibition assay for the detection of phospho USP7 (Ser18).
Key Applications
  • Western Blotting
  • Peptide Inhibition Assay
Application NotesPeptide Inhibition Analysis: 0.2 µg/mL from a representative lot was blocked by a phospho-peptide in HeLa cell lysate.

Western Blotting Analysis: 0.02 µg/mL from a representative lot detected phospho USP7 (Ser18) in HeLa cell lysate (Prof. Grigory Dianov, University of Oxford, U.K.).

Western Blotting Analysis: 0.02 µg/mL from a representative lot detected 100 ng of phospho USP7 (Ser18), and not dephosphorylated USP7 (Prof. Grigory Dianov, University of Oxford, U.K.).

Alexa Fluor® is a registered trademark of Life Technologies®.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a region near the N-terminus of human USP7 phosphorylated at Ser18.
EpitopeNear N-terminus
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Porcine, and Xenopus based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • USP7
  • HAUSP
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~140 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected phospho USP7 (Ser18) in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC225 04053252958151