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05-1143 Anti-phospho-Paxillin (Tyr31) Antibody, clone M102

Detect phospho-Paxillin (Tyr31) using this Anti-phospho-Paxillin (Tyr31) Antibody, clone M102 validated for use in WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: FLJ16691, paxillin UniProt Number: P49023
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05-1143
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1143
ReplacesMAB1146
DescriptionAnti-phospho-Paxillin (Tyr31) Antibody, clone M102
Background InformationPaxillin is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and an SH3- and SH2-binding domain that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Tyrosine phosphorylation of paxillin occurs in response to growth factors, neuropeptides, and integrins. The major sites of tyrosine phosphorylation include Tyr31 and Tyr118. Both of these sites may be involved in Crk binding to paxillin during integrin-mediated cell adhesion. These sites may provide docking motifs for recruitment of other signaling molecules to focal adhesions.
References
Product Information
FormatPurified
Control
  • Pervanadate treated L6 cell lysates
PresentationPurified monoclonal IgG1 in phosphate-buffered saline, 50% glycerol, 1 mg/mL BSA, and 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect phospho-Paxillin (Tyr31) using this Anti-phospho-Paxillin (Tyr31) Antibody, clone M102 validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenClone M102 was generated from phospho-Paxillin (Tyr31) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 31 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin.
CloneM102
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThe antibody detects Paxillin phosphorylated at Tyr31.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FLJ16691
  • paxillin
Modifications
  • Phosphorylation
Purification MethodProtein A chromatography
UniProt Number
UniProt SummaryFUNCTION: Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
SUBUNIT STRUCTURE: Binds in vitro to vinculin as well as to the SH3 domain of c-SRC and, when tyrosine phosphorylated, to the SH2 domain of V-CRK. Isoform beta binds to focal adhesion kinase but weakly to vinculin. Isoform gamma binds to vinculin but only weakly to focal adhesion kinase. Interacts with GIT1, NUDT16L1/SDOS, PARVA and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1 By similarity. Binds ASAP2. Interacts with unphosphorylated ITGA4. Interacts with RNF5.
SUBCELLULAR LOCATION: Cytoplasm › cytoskeleton. Cell junction › focal adhesion.
PTM: Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens.
SEQUENCE SIMILARITIES: Belongs to the paxillin family.
Contains 4 LIM zinc-binding domains.
Molecular Weight72 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by western Blot in pervanadate treated L6 cell lysates.
Western Blot Analysis: 1:500 dilution of this lot detected phospho-Paxillin on 10 µg of pervanadate treated L6 lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C for one year from date of receipt. Do not aliquot.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-1143 04053252618154

Documentation

Anti-phospho-Paxillin (Tyr31) Antibody, clone M102 SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-Paxillin (Tyr31) Antibody, clone M102 Certificates of Analysis

TitleLot Number
Anti-phospho-Paxillin (Tyr31), -2518470 2518470
Anti-phospho-Paxillin (Tyr31), -2779133 2779133
Anti-phospho-Paxillin (Tyr31), clone M102 - 2204944 2204944
Anti-phospho-Paxillin (Tyr31), clone M102 - 3868373 3868373
Anti-phospho-Paxillin (Tyr31), clone M102 - NG1727629 NG1727629
Anti-phospho-Paxillin (Tyr31), clone M102 - NG1842848 NG1842848
Anti-phospho-Paxillin (Tyr31), clone M102 - NG1915716 NG1915716
Anti-phospho-Paxillin (Tyr31), clone M102 - NRG1701185 NRG1701185
Anti-phospho-Paxillin (Tyr31), clone M102 Monoclonal Antibody 3131889
Anti-phospho-Paxillin (Tyr31), clone M102 Monoclonal Antibody 3068665