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ABS1004 Anti-phospho-NKCC1 Antibody (Thr212/Thr217)

Anti-phospho-NKCC1 Thr212/Thr217 is an antibody against phospho-NKCC1 for use in Western Blotting, Immunofluorescence.

Anti-phospho-NKCC1 Antibody (Thr212/Thr217) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: SLC12A2, NKCC1 UniProt Number: P55011
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ABS1004
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IFRbSerumPolyclonal Antibody
Description
Catalogue NumberABS1004
DescriptionAnti-phospho-NKCC1 Antibody (Thr212/Thr217)
Alternate Names
  • phospho-NKCC1 Thr212/Thr217
  • Solute carrier family 12 member 2
  • Basolateral Na-K-Cl symporter
  • Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1
Background InformationThe Na-K-Cl cotransporter 1 (NKCC1) is a membrane protein coded by the SLC12A2 gene and functions in cellular sodium/potassium/chloride transport and homeostasis. It is widely distributed throughout the body and serves important functions, especially in liquid-secreting glands. Phosphorylation of Thr212/Thr217 in the intracellular N-terminal domain of NKCC1 is correlated with NKCC1 activation.
References
Product Information
FormatSerum
PresentationRabbit polyclonal serum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-NKCC1 Thr212/Thr217 is an antibody against phospho-NKCC1 for use in Western Blotting, Immunofluorescence.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesWestern Blotting Analysis: A representative lot detected phospho-NKCC1 Thr212/Thr217 in GMB43 and GBM5 cell lysate (Dr. Anders Persson, UCSF) (Grim, R.P., et al. (2012). JBC. 1-31).
Immunofluorescence Analysis: A representative lot detected phospho-NKCC1 Thr212/Thr217 in GBM43 human tissiue (Dr. Anders Persson, UCSF) (Grim, R.P., et al. (2012). JBC. 1-31).
Biological Information
ImmunogenLinear peptide corresponding to human phospho-NKCC1 Thr212/Thr217.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificitySpecific for mature form of phosphorylated NKCC1.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SLC12A2
  • NKCC1
Purification MethodUnpurified
UniProt Number
Molecular Weight~250 kDa observed. Likely a dimer of mature form of NKCC1, NKCC1 is glycosylated differently in different cells, leading to uncharacterized band(s) in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in GMB43 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-NKCC1 Thr212/Thr217 in 10 µg of GMB43 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS1004 04055977133332

Documentation

Anti-phospho-NKCC1 Antibody (Thr212/Thr217) SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-NKCC1 Antibody (Thr212/Thr217) Certificates of Analysis

TitleLot Number
Anti-phospho-NKCC1 Thr212/Thr217 - 3224588 3224588
Anti-phospho-NKCC1 Thr212/Thr217 - 3474199 3474199
Anti-phospho-NKCC1 Thr212/Thr217 - 3726147 3726147
Anti-phospho-NKCC1 Thr212/Thr217 - 3909656 3909656
Anti-phospho-NKCC1 Thr212/Thr217 - 4045821 4045821
Anti-phospho-NKCC1 Thr212/Thr217 - 4123894 4123894
Anti-phospho-NKCC1 Thr212/Thr217 - 4149291 4149291
Anti-phospho-NKCC1 Thr212/Thr217 - 4216369 4216369
Anti-phospho-NKCC1 Thr212/Thr217 -2634209 2634209
Anti-phospho-NKCC1 Thr212/Thr217 -2645985 2645985
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References

Reference overviewPub Med ID
The Journal of endocrinology
Tiptaft, EM; Jones, MT
The Journal of endocrinology  64  9P-10P  1975

163881 163881

Brochure

Title
NPI Flyer- Epigenetics and Nuclear Function Feature
New Products - Antibodies, Small Molecule, Inhibitors

Technical Info

Title
White Paper: Further considerations of antibody validation and usage.