Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABE1847 Anti-phospho Mis18-binding protein 1 (Thr702) Antibody

Anti-phospho Mis18-binding protein 1 (Thr702) Antibody is an antibody against phospho Mis18-binding protein 1 for use in Immunocytochemistry, Dot Blot, Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: MIS18BP1, C14orf106, KIAA1903, KNL2, M18BP1 UniProt Number: Q6P0N0
View Products on Sigmaaldrich.com
ABE1847
100 μg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, DB, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1847
DescriptionAnti-phospho Mis18-binding protein 1 (Thr702) Antibody
Alternate Names
  • Mis18-binding protein 1
  • phospho Mis18-binding protein 1 (Thr702)
  • Kinetochore null 2 homolog
  • Kinetochore-associated protein KNL-2 homolog
  • hsKNL-2
  • P243
  • Putative protein p243 which interacts with transcription factor Sp1
Background InformationMis18-binding protein 1 (UniProt Q6P0N0; also known as hsKNL-2, Kinetochore null 2 homolog, Kinetochore-associated protein KNL-2 homolog, P243, Putative protein p243 which interacts with transcription factor Sp1) is encoded by the MIS18BP1 (also known as C14orf106, KIAA1903, KNL2, M18BP1) gene (Gene ID 55320) in human. Mis18-binding protein 1 is a component of the Mis18 complex (Mis18α, Mis18β, and M18BP1/KNL2) that plays a role in mediating cell cycle-dependent deposition/incorporation of the histone H3 variant CENP-A at the centromeres. CENP-A incorporation is carried out by at least two sets of events, the Mis18 complex must first localize to centromeres at the onset of anaphase, HJURP-mediated CENP-A deposition then occurs during G1. Polo-like kinase 1 (Plk1) is shown to phosphorylate M18BP1 on Thr702 and plays an important role in promoting Mis18 complex centromere localization.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 30% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-phospho Mis18-binding protein 1 (Thr702) Antibody is an antibody against phospho Mis18-binding protein 1 for use in Immunocytochemistry, Dot Blot, Western Blotting.
Key Applications
  • Immunocytochemistry
  • Dot Blot
  • Western Blotting
Application NotesImmunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected phospho Mis18-binding protein 1 (Thr702) in HeLa cells.
Dot Blot Analysis: 1 µg/mL from a representative lot detected the immunogen peptide, but not the corresponding non-phosphorylated sequence, by dot blot analysis.
Immunocytochemistry Analysis: A representative lot detected Thr702 phosphorylated Mis18-binding protein 1/M18BP1 localized at the centromeres in HeLa cells (McKinley, K.L., and Cheeseman, I.M. (2014). Cell. 158(2):397-411).
Western Blotting Analysis: A representative lot detected in vitro phosphorylation of recombinant Mis18-binding protein 1/M18BP1 on Thr702 by recombinant polo-like kinase 1 (Plk1) (McKinley, K.L., and Cheeseman, I.M. (2014). Cell. 158(2):397-411).
Biological Information
ImmunogenLinear peptide corresponding to a human M18BP1 sequence containing phosphorylated Thr702.
EpitopeM18BP1 pThr702
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificitySpecifically reacts with the immunogen peptide, but not the corresponding non-phosphorylated sequence, by dot blot analysis. Phosphorylation site is not present in the spliced isoform 2 of human Mis18-binding protein 1/M18BP1.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MIS18BP1
  • C14orf106
  • KIAA1903
  • KNL2
  • M18BP1
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight129 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: 4.0 µg/mL of this antibody detected phospho Mis18-binding protein 1 (Thr702) in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1847 04055977312553