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MABS155 Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1

Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1 is an antibody against phospho-GRK2 (Ser670) for use in Western Blotting, Immunoprecipitation, Peptide Inhibition Assay.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Hormones & Receptors Gene Symbol: ADRBK1, BARK, BARK1, GRK2 UniProt Number: P25098
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MABS155
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IP, PIAMUnconjugatedMonoclonal Antibody
Description
Catalogue NumberMABS155
DescriptionAnti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1
Alternate Names
  • Beta-adrenergic receptor kinase 1
  • Beta-ARK-1
  • G-protein coupled receptor kinase 2
Background InformationG-protein coupled receptor kinase 2 (Grk2), also known as BARK or BARK1, is a member of the large family of GRK kinases that phosphorylate G-protein coupled receptors (GPCR), resulting in the recruitment of arrestins to the cytoplasmic domains of GPCR’s and subsequent internalization of these receptors by endocytosis. GRK2 is ubiquitously expressed, and modulates diverse cellular processes through association with a number of non-receptor substrates. GRK2 regulates actin dynamics and cell motililty through interaction with radixin, tubilin, and ezrin. In addition, Grk2 may play a role in the signaling of TGFβ by blocking the nuclear translocation of Smad2 and Smad3 proteins. It has also been shown that Grk2 may play a role in the hedgehog signaling pathway during embryonic development, by modulating the signaling of the atypical G-protein coupled receptor Smo. Grk2 is inactivated by phosphorylation of ser670 by ERK. It is also phosphorylated by PKC and Src, depending on the cellular context.
References
Product Information
FormatUnconjugated
Control
  • Untreated and Calyculin A/Okadaic Acid treated A431 cells
PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1 is an antibody against phospho-GRK2 (Ser670) for use in Western Blotting, Immunoprecipitation, Peptide Inhibition Assay.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Peptide Inhibition Assay
Application NotesPeptide Inhibition Assay Analysis: A 1:1,000 dilution from a representative lot blocked phospho-GRK2 in 10 µg of Calyculin A/Okadaic Acid treated and untreated Jurkat cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated phospho-GRK2 in HeLa cell lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human GRK2 phosphorylated at Ser670.
EpitopePhosphorylated Ser670
Clone15G11.1
HostMouse
SpecificityThis antibody recognizes GRK2 phosphorylated at Ser670.
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ADRBK1
  • BARK
  • BARK1
  • GRK2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~70 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in untreated and Calyculin A/Okadaic Acid treated A431 cells.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected GRK2 in 10 µg of untreated and Calyculin A/Okadaic Acid treated A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS155 04053252440854

Documentation

Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho-GRK2 (Ser670) Antibody, clone 15G11.1 Certificates of Analysis

TitleLot Number
Anti-phospho-GRK2 (Ser670), -2576404 2576404
Anti-phospho-GRK2 (Ser670), -2640559 2640559
Anti-phospho-GRK2 (Ser670), -2686168 2686168
Anti-phospho-GRK2 (Ser670), -2718264 2718264
Anti-phospho-GRK2 (Ser670), -2792488 2792488
Anti-phospho-GRK2 (Ser670), clone 15G11.1 - 2298450 2298450
Anti-phospho-GRK2 (Ser670), clone 15G11.1 - 3423237 3423237
Anti-phospho-GRK2 (Ser670), clone 15G11.1 - 4162702 4162702
Anti-phospho-GRK2 (Ser670), clone 15G11.1 - Q2032354 Q2032354
Anti-phospho-GRK2 (Ser670), clone 15G11.1 Monoclonal Antibody 3050683