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MABS1280-25UG Anti-mS2i6A Antibody, clone CL-6

Anti-mS2i6A, clone CL-6 Antibody, Cat. No. MABS1280-25UG, validated for use in Dot Blot and ELISA.

Research Category: Epigenetics & Nuclear Function UniProt Number: N/A
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MABS1280-25UG
25 μg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MaDB, ELISAMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1280-25UG
DescriptionAnti-mS2i6A Antibody, clone CL-6
Alternate Names
  • N6-isopenetyl-2-thiomethyladenosine
Background InformationThe mS2i6A is an evolutionally conserved modification found in the tRNA. The hypermodified mS2i6A at position 37 occurs frequently in tRNAs that read codons starting with uridine. The mS2 group of mS2i6A is critical for the accurate decoding of Tyr and Phe codons. It has been shown that a deficiency of mS2i6A in the cognate poly(U) reader tRNA(Phe) leads to increased misreading when the near-cognate competitor tRNA4(Leu) is wild-type. It can lead to higher rejection rate of tRNA4(Leu) by more aggressive proofreading on the wild-type ribosome. It is believed that Cdk5rap1 specifically catalyzes the mS2 modification of mt-tRNAs and contributes to mitochondrial function in vivo. This modification regulates efficient mitochondrial translation and energy metabolism in mammals. Cdk5rap1 knockout mice that are deficient in the 2-methylthio modification in mt-tRNAs bearing Ser(UCN), Phe, Tyr and Trp, impaired mitochondrial protein synthesis is observed, which causes respiratory defects of mitochondria and myopathy in vivo. (Ref.: Deuchler, M et al. (2016). Cell Mol Life Sci. 73 (16); 3075-3095; Fakruddin, M., et al. (2017). Nucleic Acids Res. 2017 Nov 16;45(20):11954-11961).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-mS2i6A, clone CL-6 Antibody, Cat. No. MABS1280-25UG, validated for use in Dot Blot and ELISA.
Key Applications
  • Dot Blot
  • ELISA
Application NotesDot Blot Analysis: A 1:200 dilution of this antibody detected mS2i6A in Mouse liver total RNA and Cdk5rap1 KO  mouse total RNA (Courtesy of Fan - Yan Wei, Ph.D ., Faculty of Life Sciences,  Kumamoto University Japan ).
Biological Information
ImmunogenA synthesized mS2i6A nucleotide conjugated to BSA.
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone CL-6 recognizes wild-type total RNA, but not Cdk5rap1 KO  total RNA.
IsotypeIgG2b
Species Reactivity
  • Mammals
Species Reactivity NoteMammals.
Antibody TypeMonoclonal Antibody
Purification MethodProtein G purified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Dot Blot in Mouse Liver Total RNA.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1280-25UG 04054839421082

Documentation