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MABS2002-25UG Anti-mPEG Antibody, clone 15-2b

Anti-mPEG, clone 15-2b, Cat. No. MABS2002, is a mouse monoclonal antibody that detects methoxy-PEG (mPEG)-conjugated proteins and has been tested for use in ELISA.

MABS2002-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS2002-25UG
DescriptionAnti-mPEG Antibody, clone 15-2b
Alternate Names
  • Methoxy polyethylene glycol
Background InformationPolyethylene glycols (PEG) are non-toxic, uncharged, hydrophilic long chain polymers with a big exclusion volume in aqueous solutions. The covalent attachment of PEG is commonly employed to modify proteins and drugs that can increase their bioavailability and reduce immunogenicity and proteolytic degradation. The monomethoxylated form of polyethylene glycol (mPEG) has been widely employed in drug modifications. mPEG modification reduces nonspecific binding and prevents recognition by the reticuloendothelial system. This monoclonal antibody (clone 15-2b) specifically binds to the terminal methoxy group, but not the backbone of PEG. It displays high affinity for the terminal methoxy group and can be easily used as a detection antibody for the quantification of mPEG by ELISA. It is also suitable for detection of PEGylated liposomes and nanoparticles. The mPEG cell-based competition ELISA can detect lower molecular-weight (< 2 kDa) mPEG molecules even at nanomolar concentration and the assay is not affected by the presence of mouse or human serum. In addition, mPEG cell-based ELISA can be useful in determining the pharmacokinetics of a mPEG molecule an animal without the need for any radiolabeling.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in PBS with 0.04% sodium azide.
Applications
ApplicationAnti-mPEG, clone 15-2b, Cat. No. MABS2002, is a mouse monoclonal antibody that detects methoxy-PEG (mPEG)-conjugated proteins and has been tested for use in ELISA.
Key Applications
  • ELISA
Biological Information
ImmunogenMethoxy PEG covalently conjugated to E.coli beta-glucuronidase.
Clone15-2b
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 15-2b specifically detects proteins conjugated with methoxy polyethylene glycol (mPEG).
IsotypeIgG2bκ
Species Reactivity
  • All
Species Reactivity NoteAll.
Antibody TypeMonoclonal Antibody
Purification MethodProtein A purified
Molecular WeightN/A
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by ELISA with methoxy Polyethylene Glycol (10KPEG, 3KPEG, and 2K mPEG).

ELISA Analysis: 5 µg/mL of this antibody detected methoxy polyethylene glycol (2K-mPEG) and with minimal reactivity with 10K and 3K amino-PEGs.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2002-25UG 04054839613630

Documentation