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05-672 Anti-dimethyl-Histone H4 (Lys20) Antibody, clone 6G7/H4

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05-672
200 µg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, H, M, RIP, WB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue Number05-672
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-dimethyl-Histone H4 (Lys20) Antibody, clone 6G7/H4
Alternate Names
  • H4K20me2
  • Histone H4 (di methyl K20)
References
Product Information
FormatPurified
PresentationPBS
Quality LevelMQ100
Applications
ApplicationUse Anti-dimethyl-Histone H4 (Lys20) Antibody, clone 6G7/H4 (mouse monoclonal antibody) validated in IP, WB, IHC to detect dimethyl-Histone H4 (Lys20) also known as H4K20me2, Histone H4 (di methyl K20).
Key Applications
  • Immunoprecipitation
  • Western Blotting
  • Immunohistochemistry
Biological Information
Immunogenpeptide corresponding to amino acids 17-28 of human histone H4 (RHRdimKVLRDNIQGC) dimethylated on lysine 20.
Cloneclone 6G7/H4
HostMouse
Specificitymethyl-Histone H4
IsotypeIgG1
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Gene Symbol
  • HIST1H4A
  • H4/J
  • HIST1H4F
  • HIST1H4L
  • H4FN
  • H4FA
  • H4FH
  • HIST1H4H
  • H4F2
  • H4FM
  • H4/A
  • H4FK
  • H4FG
  • HIST2H4
  • H4FB
  • H4/K
  • HIST1H4I
  • H4/N
  • HIST1H4B
  • H4FE
  • H4/M
  • H4/a
  • HIST1H4E
  • HIST4H4
  • HIST2H4A
  • H4FC
  • H4FI
  • H4/H
  • HIST1H4C
  • H4/G
  • HIST1H4K
  • H4FJ
  • H4FD
  • H4/I
  • H4/B
  • H4/D
  • H4/C
  • HIST1H4J
  • HIST1H4D
  • H4/E
Modifications
  • Methylation
Purification MethodProtein G Chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 103 amino acids; 11367 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
Molecular Weight10kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on HeLa acid extracts
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions2 years at -20°C
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-672 04053252275807

Documentation

Anti-dimethyl-Histone H4 (Lys20) Antibody, clone 6G7/H4 SDS

Title

Safety Data Sheet (SDS) 

Anti-dimethyl-Histone H4 (Lys20) Antibody, clone 6G7/H4 Certificates of Analysis

TitleLot Number
Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 (mouse monoclonal IgG1) - 2138533 2138533
Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 (mouse monoclonal IgG1) - 2384632 2384632
Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 (mouse monoclonal IgG1) - 1990542 1990542
Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 (mouse monoclonal IgG1) - 1995610 1995610
Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 (mouse monoclonal IgG1) - 2066050 2066050
Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 (mouse monoclonal IgG1) - 2280286 2280286
Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 - 3948768 3948768
Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 -2536303 2536303
Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 -2580689 2580689
Anti-dimethyl-Histone H4 (K20), clone 6G7/H4 -2601715 2601715

References

Reference overviewPub Med ID
RNF8- and RNF168-dependent degradation of KDM4A/JMJD2A triggers 53BP1 recruitment to DNA damage sites.
Frédérick A Mallette,Francesca Mattiroli,Gaofeng Cui,Leah C Young,Michael J Hendzel,Georges Mer,Titia K Sixma,Stéphane Richard
The EMBO journal  31  2012

Show Abstract
22373579 22373579
PR-Set7 is a nucleosome-specific methyltransferase that modifies lysine 20 of histone H4 and is associated with silent chromatin.
Nishioka, Kenichi, et al.
Mol. Cell, 9: 1201-13 (2002)  2002

Show Abstract
12086618 12086618

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Life Science Research > Antibodies and Assays > Primary Antibodies