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ABE441 Anti-dimethyl Histone H4 (Arg3), Asymmetric Antibody

This Anti-dimethyl Histone H4 (Arg3) antibody, Asymmetric is validated for use in western blotting, ICC & dot blot for the detection of dimethyl Histone H4 (Arg3).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H4A, H4/A, H4FA, HIST1H4B, H4/I, H4FI, HIST1H4C, H4/G, H4FG, HIST1H4D, H4/B, H4FB, HIST1H4E, H4/J, H4FJ, HIST1H4F, H4/C, H4FC, HIST1H4H, H4/H, H4FH, HIST1H4I, H4/M, H4FM, HIST1H4J, HIST4/E, H4FE, HIST1H4K, H4/D, H4FD, HIST1H4L, H4/K, H4FK, HIST2H4A, H4/N, H4F2, H4FN, HIST2H4, HIST2H4B, H4/O, H4FO, HIST4H4 UniProt Number: P62805
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ABE441
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, DBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE441
DescriptionAnti-dimethyl Histone H4 (Arg3), Asymmetric Antibody
Alternate Names
  • Histone H4
Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The histones have an amino terminal tail, a globular domain, and a carboxy-terminal tail. The four core histones, H2A, H2B, H3 and H4 assemble into the octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped in a sequence-independent manner around the octamer, forming the basic subunit of chromatin, the nucleosome. Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-dimethyl Histone H4 (Arg3) antibody, Asymmetric is validated for use in western blotting, ICC & dot blot for the detection of dimethyl Histone H4 (Arg3).
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Dot Blot
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected dimethyl Histone H4 (Arg3), Asymmetric in HeLa cells.

Dot Blot (Specificity) Analysis: A 1:10,000 dilution from a representative lot detected dimethyl Histone H4 (Arg3), Asymmetric in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

DyLight® is a registered trademark of Thermo Fisher Scientific.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Histone H4, asymmetrically dimethylated at Arg3.
EpitopeN-terminus
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H4A
  • H4/A
  • H4FA
  • HIST1H4B
  • H4/I
  • H4FI
  • HIST1H4C
  • H4/G
  • H4FG
  • HIST1H4D
  • H4/B
  • H4FB
  • HIST1H4E
  • H4/J
  • H4FJ
  • HIST1H4F
  • H4/C
  • H4FC
  • HIST1H4H
  • H4/H
  • H4FH
  • HIST1H4I
  • H4/M
  • H4FM
  • HIST1H4J
  • HIST4/E
  • H4FE
  • HIST1H4K
  • H4/D
  • H4FD
  • HIST1H4L
  • H4/K
  • H4FK
  • HIST2H4A
  • H4/N
  • H4F2
  • H4FN
  • HIST2H4
  • HIST2H4B
  • H4/O
  • H4FO
  • HIST4H4
Modifications
  • Methylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~13 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in untreated and CARM1 treated recombinant Histone H4.

Western Blotting Analysis: A 1:50,000 dilution of this antibody detected dimethyl Histone H4 (Arg3), Asymmetric in 0.5 µg of CARM1 treated recombinant Histone H4.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE441 04053252951961