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ABS2252-25UL Anti-ZnT8

Anti-ZnT8, Cat. No. ABS2252, is a rabbit polyclonal antibody that detects Zinc transporter 8 (ZnT8) and is tested in Immunofluorescence.

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ABS2252-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue NumberABS2252-25UL
DescriptionAnti-ZnT8
Alternate Names
  • Zinc transporter 8
  • Solute carrier family 30 member 8
Background InformationZinc transporter 8 (UniProt: Q8IWU4; also known as ZnT-8, Solute carrier family 30 member 8) is encoded by the SLC30A8 (also known as ZNT8) gene (Gene ID: 169026) in human. Zinc is required for insulin crystallization, storage, and secretion in the secretory granules of pancreatic β-cells. Delivery of Zn2+ into the insulin-containing granules is largely accomplished by the ZnT8 protein. ZnT8 is a homodimeric, multi-pass membrane protein with four cytoplasmic domains, six transmembrane domains, and three extracellular domains. It serves as a granule-specific Zn2+ transporter that is abundantly expressed in pancreatic islet cells. It contains a histidine-rich region, HXXXXXHNH-motif, in its third cytoplasmic domain, which serves as a ligand for zinc. ZnT8 is expressed in both a and b cells, but not in  cells. It plays a major role in Zn2+ uptake into the secretory granules. During stimulated secretion, Zn2+ is shown to be co-released with other granular content into the extracellular medium.
Loss of ZnT8 function is reported to reduce total islet Zn2+ content as well as free Zn2+ in the cytosol and granules. Loss of ZnT8 also results in reduced dense core granules and increased granule diameter. Studies have shown that the expression of ZnT8 is significantly reduced in the islets of diabetic murine models. Two isoforms of ZnT8 have been described that are produced by alternative splicing. (Ref.: Ghazvini Zadeh, EH., et al. (2020). Cell Reports 32(2); 107904; Dwivedi, OP., et al. (2019). Nat. Genetics. 51(11); 1596-1606).
References
Product Information
FormatSerum
PresentationRabbit polyclonal antibody in 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-ZnT8, Cat. No. ABS2252, is a rabbit polyclonal antibody that detects Zinc transporter 8 (ZnT8) and is tested in Immunofluorescence.
Key Applications
  • Immunofluorescence
Application NotesTested Applications

Immunofluorescence Analysis: A representative lot detected ZnT8 in Immunofluorescence applications (Zadeh, EHG., et.al. (2020). Cell Rep. 32(2):107904).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 102 amino acids from the C-terminal, cytoplasmic domain of human zinc transporter 9 (ZnT8).
EpitopeC-terminus
ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Zinc transporter 8 (ZnT8). It targets an epitope within the C-terminal, cytoplasmic domain.
IsotypeIgG
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • SLC30A8
  • ZNT8
Purification MethodUnpurified
UniProt Number
Molecular Weight40.76 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunofluorescence in mouse pancreas frozen formalin fixed sections.

Immunofluorescence Analysis: A 1:100 dilution of this antibody detected ZnT8 in mouse pancreas frozen formalin fixed sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS2252-25UL 04065266298291