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MABT339 Anti-ZO-1 Antibody, clone 5G6.1

This Anti-ZO-1 Antibody, clone 5G6.1 is validated for use in Western Blotting, Immunocytochemistry, Flow Cytometry, ELISA for the detection of ZO-1 .

Anti-ZO-1 Antibody, clone 5G6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: TJP1, ZO1 UniProt Number: Q07157
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MABT339
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT339
DescriptionAnti-ZO-1 Antibody, clone 5G6.1
Alternate Names
  • Tight junction protein ZO-1
  • Tight junction protein 1
  • Zona occludens protein 1
  • Zonula occludens protein 1
  • ZO-1
Background InformationTight junction protein ZO-1 (UniProt Q07157; also known as Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1) is encoded by the TJP1 gene (also known as ZO1) (Gene ID 7082) in human. The tight junction (TJ) constitutes the barrier between the apical and the basolateral domains of the plasma membrane. The assembly and permeability of this barrier are dependent on the zonula occludens (ZO) membrane-associated guanylate kinase (MAGUK) proteins ZO-1, ZO-2, and ZO-3. ZO-1, a 210-225 kDa protein, is found at the submembranous domain of TJs in epithelia and endothelia. ZO-1 contains three PDZ domains (PDZ1/aa23-109; PDZ2/aa181-261; PDZ3/aa422-502) at its N-treminal end, followed by an SH3 domain (aa519-580), a GUK/GK homology domain (aa632-782), an acidic domain (aa817-894), an alpha spliced domain (aa921-1000), and a C-terminal proline-rich/PR domain. ZO-1 is a phosphoprotein and a known substrate of serine/threonine kinases ZAK and of PKC. MAPK signaling pathway regulates tyrosine phosphorylation of ZO-1, and MEK1 inhibition in Ras transformed epithelial cells is reported to result in tyrosine phosphorylation of ZO-1 and occludin. ZO-1 interacts with claudins, JAM, ZO-2, and ZO-3 through its PDZ domains, while its GK module mediates interaction with occludin. ZO-1 also binds actin cytoskeleton and actin-binding protein 4.1 through its carboxyl terminal end. In addition, ZO-1 associates with AF-6 and cingulin at the TJ, as well as with the adherens junction protein alpha-catenin and with the gap junction proteins connexins 43 and 45.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-ZO-1 Antibody, clone 5G6.1 is validated for use in Western Blotting, Immunocytochemistry, Flow Cytometry, ELISA for the detection of ZO-1 .
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected ZO-1 in human and rat kidney tissues.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human ZO-1.
Clone5G6.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TJP1
  • ZO1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~195 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ZO-1 in 200 µg of HCT116 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT339 04055977173505

Documentation

Anti-ZO-1 Antibody, clone 5G6.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-ZO-1 Antibody, clone 5G6.1 Certificates of Analysis

TitleLot Number
Anti-ZO-1, clone 5G6.1 - 3219908 3219908
Anti-ZO-1, clone 5G6.1 - 3274285 3274285
Anti-ZO-1, clone 5G6.1 - 3590146 3590146
Anti-ZO-1, clone 5G6.1 - 3744303 3744303
Anti-ZO-1, clone 5G6.1 - 3891833 3891833
Anti-ZO-1, clone 5G6.1 - 3923541 3923541
Anti-ZO-1, clone 5G6.1 - 3958817 3958817
Anti-ZO-1, clone 5G6.1 - 3988493 3988493
Anti-ZO-1, clone 5G6.1 - 4023307 4023307
Anti-ZO-1, clone 5G6.1 - 4044216 4044216
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Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.