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MABE1906-100UL Anti-Venus (YFP) Antibody, clone 5

Anti-Venus (YFP), clone 5, Cat. No. MABE1906, is a mouse monoclonal antibody that detects proteins labeled with mVenus and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

MABE1906-100UL
100 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABE1906-100UL
DescriptionAnti-Venus (YFP) Antibody, clone 5
Alternate Names
  • GFP(YFP)
  • GFP(F46L)
Background InformationGreen fluorescent protein (UniProt: P42212; also known as GFP) is encoded by the GFP gene in Aequorea victoria (Jelly Fish). GFP is a 27 kDa protein from jellyfish that serves as an energy-transfer acceptor. It transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer and it fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. GFP has an absorption max at 395 nm and the emission peaks at 507 to 510 nm. However, the exact value of emission max depends on the environment of the chromophore. GFP has evolved into an important tool in cell biology research that displays stability under fixation conditions and is suitable for a variety of applications. Venus is a genetic mutant with F46L substitution in green fluorescent protein (GFP), derived from Aequorea victoria. Its excitation/emission peaks are 515/528 nm respectively and is often used as a donor-acceptor pair by researchers in FRET based co-localization studies. Compared to other fluorescent tags, Venus provides higher expression levels and better yields in preparative purifications. A fusion protein consisting of Venus and a defective mutant of p27 protein (p27K ) has been used to identify and isolate a population of quiescent cells and visualize the G0 to G1 transition. Clone 5 specifically detects only the Venus-tagged proteins. (Ref.: Nagai, T., et al. (2002). Nat. Biotechnol. 20(1); 87-90; Oki, T., et al. (2014). Sci. Rep. 4; Article 4012; Rana, MS., et al. (2018). Biochemistry 57(49); 6741-6751).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Venus (YFP), clone 5, Cat. No. MABE1906, is a mouse monoclonal antibody that detects proteins labeled with mVenus and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunoprecipitation
Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Venus (YFP) in NIH3T3 (L20V-9-11) cells (Courtesy of Li Ten and Kensaku Murano, Ph.D., Keio University School of Medicine, Tokyo Japan).

Western Blotting Analysis: A 1:250 dilution from a representative lot detected Venus (YFP) in HEK293T cells transfected with mVenus that was expressed using pCAGGS-Myc eukaryotic expression plasmid (Courtesy of Li Ten and Kensaku Murano, Ph.D., Keio University School of Medicine, Tokyo Japan).

Immunoprecipitation Analysis: A 1:1,000 dilution from a representative lot immunoprecipitated Venus (YFP) labeled proteins in pCAGGS-myc (negative) and pCAGGS-myc-mVenus (positive) HEK293T cell lysates (Courtesy of Li Ten and Kensaku Murano, Ph.D., Keio University School of Medicine, Tokyo Japan).
Biological Information
ImmunogenGST‐tagged full-length mVenus, a genetic mutant of GFP derived from Aequorea Victoria (Jelly fish).
EpitopeUnknown
Clone5
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 5 is a mouse monoclonal antibody that detects proteins labeled with Venus (YFP). It does not display cross-reactivity with mCherry, mRuby, or mClover.
IsotypeIgG1κ
Species Reactivity
  • All
Species Reactivity NoteAequorea victoria. Predicted to react with all species.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodProtein G purified
UniProt Number
Molecular Weight~35 kDa observed; 26.88 kDa calculated for GFP. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1906-100UL 04065265237765

Documentation

Anti-Venus (YFP) Antibody, clone 5 SDS

Title

Safety Data Sheet (SDS) 

Anti-Venus (YFP) Antibody, clone 5 Certificates of Analysis

TitleLot Number
Anti-Venus (YFP), clone 5 - 3754759 3754759
Anti-Venus (YFP), clone 5 - 3810045 3810045
Anti-Venus (YFP), clone 5 - 3814877 3814877
Anti-Venus (YFP), clone 5 - 3821579 3821579
Anti-Venus (YFP), clone 5 - 3837741 3837741
Anti-Venus (YFP), clone 5 - 3849523 3849523
Anti-Venus (YFP), clone 5 - 3855720 3855720
Anti-Venus (YFP), clone 5 - 3860871 3860871
Anti-Venus (YFP), clone 5 - 3863376 3863376
Anti-Venus (YFP), clone 5 - 3923597 3923597
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