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MAB3757-I-25UL Anti-VEGF Receptor-3 Antibody, clone 9D9F9

Anti-VEGF Receptor-3, clone 9D9F9, Cat. No. MAB3757-I, is a mouse monoclonal antibody that detects VEGF receptor 3 and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

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MAB3757-I-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue NumberMAB3757-I-25UL
DescriptionAnti-VEGF Receptor-3 Antibody, clone 9D9F9
Alternate Names
  • Vascular endothelial growth factor receptor 3
  • EC:2.7.10.1
  • Fms-like tyrosine kinase 4
  • FLT-4
  • Tyrosine-protein kinase receptor FLT4
Background InformationVascular endothelial growth factor receptor 3 (UniProt: P35916; also known as EC:2.7.10.1, VEGFR-3, Fms-like tyrosine kinase 4, FLT-4, Tyrosine-protein kinase receptor FLT4) is encoded by the FLT4 (also known as VEGFR3) gene (Gene ID: 2324) in human. FLT-4 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-775), a transmembrane domain (aa 776-796), and cytoplasmic domain (aa 797-1363). It is expressed in endothelial cells and is also detected in fetal spleen, lung, and brain. In adults it is detected in liver, muscle, thymus, placenta, lung, testis, ovary, prostate, heart, and kidney. FLT-4 has tyrosine-protein kinase activity and acts as a receptor for VEGFC and VEGFD. It plays a role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Its secreted form functions as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Its major function is to promote proliferation, survival, and migration of endothelial cells, and regulates angiogenic sprouting. FLT-4 undergoes trans auto-phosphorylation on tyrosine residues upon ligand binding. Phosphorylation at tyrosine 1068 is required for autophosphorylation at additional tyrosine residues. Phosphorylation at tyrosine 1063 and 1337 are important for interaction with Crk adaptor proteins and subsequent activation of MAPK8. In addition, its phosphorylation at tyrosine 1230, 1231, and 1337 are reported to be important for interaction with Grb2 and subsequent activation of the AKT1 and ERK1/ERK2 signaling pathways.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-VEGF Receptor-3, clone 9D9F9, Cat. No. MAB3757-I, is a mouse monoclonal antibody that detects VEGF receptor 3 and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunoprecipitation
  • Immunohistochemistry (Paraffin)
  • ELISA
Application NotesTested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated VEGF Receptor-3 in Immunoprecipitation applications (Bando, H., et al. (2004). Int J Cancer; 111(2):184-91).

ELISA Analysis: A representative lot detected VEGF Receptor-3 in ELISA applications (Bando, H., et al. (2004). Int J Cancer; 111(2):184-91).

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected VEGF Receptor-3 in human placenta and human kidney tissue sections.

Western Blotting Analysis: A representative lot detected VEGF Receptor-3 in Western Blotting applications (Bando, H., et al. (2004). Int J Cancer; 111(2):184-91; Zhang, Y., et al. (2018). Nat Commun.; 9(1):1296).

Immunohistochemistry Applications: A representative lot detected VEGF Receptor-3 in Immunohistochemistry applications (Bando, H., et al. (2004). Int J Cancer; 111(2):184-91; Jussila, L. et al. (1998). Cancer Res.; 58(8):1599-604; Su, C., et al. (2011). J Exp Clin Cancer Res.; 30(1):85; Valtola, R., et al. (1999). Am J. Pathol.; 154(5):1381-90).

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected VEGF Receptor-3 in human umbilical vascular endothelial cells (HUVEC).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenA recombinant fragment corresponding to the extracellular domain of human Vascular endothelial growth factor receptor 3 (VEGFR-3).
EpitopeExtracellular domain
Clone9D9F9
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 9D9F9 is a mouse monoclonal antibody that detects Vascular endothelial growth factor receptor 3 (VEGFR-3). It targets an epitope within the extracellular domain.
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FLT4
Purification MethodProtein G purified
UniProt Number
Molecular Weight~190 kDa observed; 152.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from HEK293 cells over-expressing VEGFR-3.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Vascular endothelial growth factor receptor 3 (VEGFR-3) in lysate from HEK293 cells over-expressing human VEGFR-3.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB3757-I-25UL 04065266876253