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ABE1900-100UG Anti-UPF3B

Anti-UPF3B, Cat. No. ABE1900, is a highly specific rabbit polyclonal antibody that targets Regulator of nonsense transcripts 3B (UPF3B) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

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ABE1900-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberABE1900-100UG
DescriptionAnti-UPF3B
Alternate Names
  • Regulator of nonsense transcripts 3B
  • Nonsense mRNA reducing factor 3B
  • hUpf3B
  • Up-frameshift suppressor 3 homolog on chromosome X
  • hUpf3p-X
Background InformationRegulator of nonsense transcripts 3B (UniProt: Q9BZI7; also known as Nonsense mRNA reducing factor 3B, Up-frameshift suppressor 3 homolog B, UPF3B, hUpf3B, Up-frameshift suppressor 3 homolog on chromosome X, hUpf3p-X) is encoded by the UPF3B (also known as RENT3B, UPF3X) gene (Gene ID: 65109) in human. UPF3B is widely expressed, including in testis, uterus, heart, muscle, brain, spinal cord, and placenta. It shuttles between the nucleus and the cytoplasm. It is involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serves as a link between the EJC core and NMD machinery. UPF3B recruits UPF2 at cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex is believed to activate NMD. In cooperation with UPF2 it stimulates both ATPase and RNA helicase activities of UPF1. Mutations in UPF3B gene have been linked to X-linked mental retardation that is characterized by significantly low general intellectual functioning associated with impairments in adaptive behavior, autistic features, poor musculature and other abnormalities. Two isoforms of UPF3B have been described that are produced by alternative splicing.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-UPF3B, Cat. No. ABE1900, is a highly specific rabbit polyclonal antibody that targets Regulator of nonsense transcripts 3B (UPF3B) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected UPF3B in lysate from HeLa cells overexpressing UPF3B. (Courtesy of Matthew Kim & Dr. Wilkinson Miles at University of California, San Diego).

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected UPF3B in human testis and mouse testis tissue sections.

Western Blotting Analysis: 2 µg/mL from a representative lot detected UPF3B in lysate from HEK cells transfected with UPF3B vector, but not in HEK cells transfected with control vector.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal half of human Up-frameshift suppressor 3 homolog B.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects human Up-frameshift suppressor 3 homolog B (UPF3B). It targets an epitope with in 18 amino acids from the C-terminal half.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • UPF3B
  • TENT3B
  • UPF3X
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~58 kDa observed; 57.76 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in iPSCs and UPF3B-KO iPSCs.

Western Blotting Analysis: 2 µg/mL of this antibody detected UPF3B in lysate from iPSCs, but not in lysate from UPF3B knockout iPSCs.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1900-100UG 04054839659881