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MABN2393-100UG Anti-TRAAK Antibody, clone N445_27

Anti-TRAAK, clone N445/27, Cat. No. MABN2393, is a mouse monoclonal antibody that detects Potassium channel subfamily K member 4 (TRAAK) and has been tested for use in Immunohistochemistry (Paraffin).

MABN2393-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABN2393-100UG
DescriptionAnti-TRAAK Antibody, clone N445_27
Alternate Names
  • Potassium channel subfamily K member 4
  • TWIK-related arachidonic acid-stimulated potassium channel protein
  • TRAAK
Background InformationPotassium channel subfamily K member 4 (UniProt: O88454; also known as TWIK-related arachidonic acid-stimulated potassium channel protein, TRAAK) is encoded by the Kcnk4 (also known as Traak) gene (Gene ID: 16528) in murine species. TRAAK is a multi-pass membrane protein that is expressed in brain, spinal cord, and eye and serves as a voltage-insensitive potassium channel. It is a mechano-activated channel that senses shear stress and negative membrane pressure. It generates progressive and non-inactivating current when negative pressure is applied to the plasma membrane. The channel opening is triggered by mechanical forces that deform the membrane. Channel opening is also triggered by raising the intracellular pH to basic levels. TRAAK channel is shown to be inactive at 24C, but raising the temperature to 37C or higher increases the frequency of channel opening. It plays a role in the sensory perception of pain caused by heat. The C-terminal of TRAAK has a charged cluster that is important in maintaining the mechanosensitive properties of the channel. TRAAK channels are reported to be gradually and reversibly activated by polyunsaturated fatty acids (PUFA) including arachidonic acid that potentiates TRAAK background currents by 5- to 20-fold. (Ref.: Noël, J., et al. (2011). Channels (Austin). 5(5); 402-409).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-TRAAK, clone N445/27, Cat. No. MABN2393, is a mouse monoclonal antibody that detects Potassium channel subfamily K member 4 (TRAAK) and has been tested for use in Immunohistochemistry (Paraffin).
Key Applications
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected TRAAK in rat brain tissue sections.
Biological Information
ImmunogenA recombinant fragment corresponding to 143 amino acids from the C-terminal, cytoplasmic domain of mouse Potassium channel subfamily K member 4 (TRAAK).
EpitopeC-terminus
CloneN445/27
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone N445/27 is a mouse monoclonal antibody that detects Potassium channel subfamily K member 4 (TRAAK). It targets an epitope within the C-terminal, cytoplasmic domain.
IsotypeIgG2aκ
Species Reactivity
  • Rat
  • Mouse
Species Reactivity NoteMouse, Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Kcnk4
  • Traak
Purification MethodProtein G purified
UniProt Number
Molecular Weight43.05 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse brain tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected TRAAK in mouse brain tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2393-100UG 04061841276722