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MABF2658-25UL Anti-TIGIT Antibody, clone 4D4

Anti-TIGIT, clone 4D4, Cat. No. MABF2658, is an Armenian hamster monoclonal antibody that detects TIGIT and is tested for use in ELISA, Flow Cytometry, Agonist Action, Inhibition Assay, and Western Blotting.

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MABF2658-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABF2658-25UL
DescriptionAnti-TIGIT Antibody, clone 4D4
Alternate Names
  • T-cell immunoreceptor with Ig and ITIM domains
  • V-set and immunoglobulin domain-containing protein 9
Background InformationT-cell immunoreceptor with Ig and ITIM domains (UniProt: P86176; also known as V-set and immunoglobulin domain-containing protein 9, TIGIT) is encoded by the Tigit (also known as Vstm3) gene (Gene ID: 100043314) in murine species. TIGIT is a single-pass type I, homodimeric, membrane protein that is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 29-148), a transmembrane domain (aa 149-169), and a cytoplasmic domain (aa 170-249). The cytoplasmic domain contains an immunoreceptor tyrosine-based inhibitor motif (ITIM; aa 234-239), which is involved in the modulation of its cellular responses. It serves as a coinhibitory receptors with CD226 and is expressed on NK cells, activated T cells, memory T cells, and a subset of regulatory T cells and blocks immune responses by affecting T cells and APCs. It is shown to bind to poliovirus receptor (PVR) with high affinity to form a heterotetrameric assembly of two TIGIT and two PVR molecules. This causes increased secretion of IL-10 and decreased secretion of IL-12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT also serves as a functional ligand and induces dendritic cells to acquire a tolerogenic phenotype via ligation of PVR. TIGIT -/- mice, when challenged, develop severe experimental autoimmune encephalomyelitis compared to wild-type mice. Loss of TIGIT also in mice also results in hyperproliferative T cell responses and increased susceptibility to autoimmunity. (Ref.: Dixon, KO., et al. (2018). J. Immunol. 200(8); 3000-3007; Joller, N., et al. (2011). J. Immunol. 186(3); 1338-1342).
References
Product Information
FormatPurified
PresentationPurified Armenian hamster monoclonal antibody IgG in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-TIGIT, clone 4D4, Cat. No. MABF2658, is an Armenian hamster monoclonal antibody that detects TIGIT and is tested for use in ELISA, Flow Cytometry, Agonist Action, Inhibition Assay, and Western Blotting.
Key Applications
  • Western Blotting
  • Agonist or Inhibitor
  • ELISA
  • Flow Cytometry
  • Inhibition
Application NotesTested Applications

ELISA Analysis: A representative lot detected TIGIT in ELISA applications (Joller, N., et al. (2011). J Immunol. 186(3):1338-42).

ELISA Analysis: Various dilutions from a representative lot detected Fc-His-tagged TIGIT chimera protein .

Flow Cytometry Analysis: A representative lot detected TIGIT in Flow Cytometry applications (Joller, N., et al. (2011). J Immunol. 186(3):1338-42).

Agonist Action: A representative lot of this antibody reduced proliferation of T cells stimulated with Anti-CD3/Anti-CD28 beads in vitro. (Dixon, K.O., et al. (2018). J Immunol. 200(8):3000-3007).

Inhibition Assay: A representative lot of this antibody fully blocked CD155 binding to TIGIT. (Dixon, K.O., et al. (2018). J Immunol. 200(8):3000-3007).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenRecombinant mouse TIGIT tetramers.
EpitopeUnknown
Clone4D4
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostArmenian Hamster
SpecificityClone 4D4 is an Armenian Hamster monoclonal antibody that detects murine T-cell immunoreceptor with Ig and ITIM domains (TIGIT).
IsotypeIgG
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Tigit
Purification MethodProtein G purified
UniProt Number
Molecular Weight~50 kDa observed; 26.96 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant mouse TIGIT-Fc chimera protein.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected recombinant mouse Fc-His-tagged TIGIT chimera protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2658-25UL 04065267780689