Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABT884 Anti-TACE Antibody, clone D1(A12)

Detect TACE/ADAM17 using this human monoclonal Anti-TACE, clone D1(A12), Cat. No. MABT884, validated for use in Flow Cytometry and Activity/Function Inhibition.

Anti-TACE Antibody, clone D1(A12) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: ADAM17, CSVP, TACE UniProt Number: P78536
View Products on Sigmaaldrich.com
MABT884
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HINHIB, FCHuman RecombinantPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT884
DescriptionAnti-TACE Antibody, clone D1(A12)
Alternate Names
  • Disintegrin and metalloproteinase domain-containing protein 17
  • ADAM 17
  • CD156b
  • Snake venom-like protease
  • TNF-alpha convertase
  • TNF-alpha-converting enzyme
Background InformationDisintegrin and metalloproteinase domain-containing protein 17 (UniProt: P78536; also known as EC:3.4.24.86, ADAM 17, Snake venom-like protease, TNF-alpha convertase, TNF-alpha-converting enzyme, TACE, CD156b) is encoded by the ADAM17 (also known as CSVP, TACE) gene (Gene ID: 6868) in human. TACE is a membrane-bound metalloprotease responsible for solubilizing many pathologically significant membrane substrates. Mature ADAM-family ectodomains contain a globular metalloprotease catalytic domain, a disulfide-dependent disintegrin cysteine rich (Dis-Cys) domain, and, in some cases, an epidermal growth factor (EGF)-like domain. ADAM17 activity is regulated by multiple post-translational modifications. It has three phosphorylation sites on its C-terminal cytoplasmic domain (T735, S791, and (S819). ERK1/2-mediated T735 phosphorylation is reported to regulate ADAM17 maturation and surface expression. TACE displays a narrow endopeptidase specificity. It cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form and is also responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Clone D1(A12) exhibits high affinity (Kd = 0.46 nM) for the TACE ectodomain and is the most selectively potent cell-surface ADAM inhibitor. It can independently bind both the complete TACE ectodomain and the isolated catalytic domain (Kd = 5.21 nM). It is shown to be about 5-fold more potent than N-TIMP3 in inhibiting cell surface TACE. Mutations in ADAM17 gene are known to cause inflammatory skin and bowel disease, neonatal, 1 (NISBD1) that is characterized by inflammatory features with neonatal onset, involving the skin, hair, and gut.
References
Product Information
FormatPurified
PresentationPurified recombinant human IgG1 in PBS without azide.
Quality LevelMQ100
Applications
ApplicationDetect TACE/ADAM17 using this human monoclonal Anti-TACE, clone D1(A12), Cat. No. MABT884, validated for use in Flow Cytometry and Activity/Function Inhibition.
Key Applications
  • Inhibits Activity/Function
  • Flow Cytometry
Application NotesInhibits Activity/Function: 6 µg/mL from a representative lot inhibited TACE-mediated CD16 shedding from the surface of stimulated primary human NK cells (Courtesy of Zachary Davis, Ph.D., University of Minnesota, USA).

Inhibits Activity/Function: Representative lots inhibited TACE-mediated substrates shedding from the surface of human cells both in cultures and in mice in vivo (Issuree, P.D., et al. (2013). J. Clin. Invest. 123(2):928-932; Richards, F.M., et al. (2012). PLoS One. 7(7):e40597; Tape, C.J., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(14):5578-5583).
Biological Information
ImmunogenSee Specificity section.
Epitopeextracellular domain
CloneD1(A12)
ConcentrationPlease refer to lot specific datasheet.
HostHuman Recombinant
SpecificityCat. No. MABT884 is a recombinant human IgG1 that contains a heavy chain variable (VH) region with affinity towards human TACE noncatalytic Dis-Cys domain and a light chain variable (VL) region that binds human TACE catalytic domain (Tape, C.J., et al. (2011). Proc. Natl. Acad. Sci. U. S. A. 108(14):5578-5583). The VH region of D1(A12) is derived from the scFv named D1 isolated from a phage library generated using pooled lymphocytes from 43 donors (Schofield, D.J., et al. (2007). Genome Biol. 8(11):R254). The D1 VH cDNA was subsequently cloned into a naïve human VL phage-display library for identification and isolation of the hybrid D1(A12) scFv with affinity toward both Dis-Cys domain and the catalytic domain. The D1 VH and A12 VL cDNAs were then cloned into a pBudCE4.1 human IgG1 expression vector for generating the recombinant antibody in HEK293 cells (Tape, C.J., et al. (2011). Proc. Natl. Acad. Sci. USA. 108(14):5578-5583).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman recombinant. Not Mouse (Kwok, H.F., et al. (2014). Protein Eng. Des. Sel. 27(6):179-190.)
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ADAM17
  • CSVP
  • TACE
UniProt Number
Molecular Weight93.02/91.12/68.22 kDa (prepro-/pro-/mature form) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in HeLa cells.

Flow Cytometry Analysis: 0.2 µg (2 µg/mL) of this antibody detected TACE expression on the surface of one million HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT884 04054839168659

Documentation

Anti-TACE Antibody, clone D1(A12) SDS

Title

Safety Data Sheet (SDS) 

Anti-TACE Antibody, clone D1(A12) Certificates of Analysis

TitleLot Number
Anti-TACE, clone D1(A12) - 3026858 3026858
Anti-TACE, clone D1(A12) - 3808649 3808649
Anti-TACE, clone D1(A12) - 3833688 3833688
Anti-TACE, clone D1(A12) - 3861912 3861912
Anti-TACE, clone D1(A12) - 3872426 3872426
Anti-TACE, clone D1(A12) - 3906473 3906473
Anti-TACE, clone D1(A12) - 4003457 4003457
Anti-TACE, clone D1(A12) - 4059298 4059298
Anti-TACE, clone D1(A12) -Q2814939 Q2814939
Anti-TACE, clone D1(A12) Monoclonal Antibody 3128144
Show more