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MABS1713 Anti-Synaptotagmin-like Protein 4 (SYTL4) Antibody, clone 7D2.2

Detect SYTL4 using this mouse monoclonal Anti-Synaptotagmin-like Protein 4 (SYTL4) Antibody, clone 7D2.2, Cat. No. MABS1713, validated for use in Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: SYTL4, SLP4 UniProt Number: Q96C24
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MABS1713
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1713
DescriptionAnti-Synaptotagmin-like Protein 4 (SYTL4) Antibody, clone 7D2.2
Alternate Names
  • Synaptotagmin-like protein 4
  • Exophilin-2
  • Granuphilin
Background InformationSynaptotagmin-like protein 4 (UniProt Q96C24; also known as Exophilin-2, Granuphilin) is encoded by the SYTL4 (also known as SLP4) gene (Gene ID 94121) in human. SYLT4 belongs to the synaptotagmin-like protein family, which contains an N-terminal RabBD (Rab-binding) domain and two C-terminal C2 domains. The characteristic feature of this family is the N-terminal domain, which is not found in other C-type tandem C2 proteins. SYLT4 is detected close to the plasma membrane and on secretory granules. Interacts with vesicles containing negatively charged phospholipids in a Ca2+-independent manner. It can bind to both the GTP- and GDP-bound forms of Rab27A and inhibits a specific GTP/GDP exchange cycle required for dense-core vesicle exocytosis. It is the first regulatory in the exocytosis pathway that functions by directly connecting Rab and SNARE. It modulates exocytosis of dense-core granules and secretion of hormones in the pancreas and the pituitary. It is not only essential for the docking of insulin granules, but also simultaneously imposes a fusion constraint on them through an interaction with syntaxin-1alpha fusion machinery.
References
Product Information
FormatPurified
PresentationPurified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect SYTL4 using this mouse monoclonal Anti-Synaptotagmin-like Protein 4 (SYTL4) Antibody, clone 7D2.2, Cat. No. MABS1713, validated for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected synaptotagmin-like protein 4 (SYTL4) in 10 µg of human adrenal gland tissue lysate.
Biological Information
ImmunogenGST-tagged recombinant human synaptotagmin-like protein 4 (SYTL4) internal fragment.
Clone7D2.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 7D2.2 epitope resides within an internal region mostly missing in human spliced isoform 2. Reactivity toward isoform 2 has not been confirmed.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SYTL4
  • SLP4
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~80 kDa observed. 70.02 kDa (human isoform 1) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected synaptotagmin-like protein 4 (SYTL4) in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1713 04054839092114